山西医科大学学报2025，Vol.56Issue(7)：776-782,7.DOI:10.13753/j.issn.1007-6611.2025.07.007
紫檀芪对脑出血大鼠的脑神经保护作用及其机制
Neuroprotective effect of Pterostilbene against cerebral hemorrhage-induced injury in rats and its underlying mechanisms
摘要
Abstract
Objective To explore the neuroprotective effects of Pterostilbene(PTE)against secondary brain injury in rats with cere-bral hemorrhage and its underlying mechanisms.Methods Sixty male rats were divided into four groups:sham group,model group,Pterostilbene group,and heme oxygenase 1 inhibitor group.The rats in Pterostilbene group were intraperitoneally injected with Pteros-tilbene at a dose of 30 mg/(kg·d)once a day for three consecutive days after modeling.The rats in inhibitor group were intraperitone-ally injected with the heme oxygenase 1 inhibitor zinc protoporphyrin(ZnPP)at a dose of 25 mg/(kg·d)at 30 min before modeling and one day after modeling,and 30 mg/(kg·d)Pterostilbene once a day for three consecutive days after modeling.The cerebral hemor-rhage model was established by stereotaxic injection of collagenase into the right striatum of the brain.The rats in sham group did not undergo modeling,and the rats in sham group and model group received equal volumes of physiological saline via intraperitoneal injec-tion once a day for three consecutive days.Subsequently,pathological changes in brain tissue were observed in the four groups using HE staining,and the modified neurological severity score(mNSS)was assessed.The water content in the affected side of brain tissue was measured by the dry-wet weight method.Neuronal apoptosis in the hematoma area of brain tissue was assessed by TUNEL method,while the serum levels of inflammatory factors(IL-1β,IL-6)were analyzed using ELISA.Additionally,Western blotting was used to measure the expressions of NF-κB signaling pathway-related proteins(p-NF-κB p65,NF-κB p65,p-IκBα,IκBα)in brain tissue.Results Compared with sham group,the disordered cellular structures were observed in the HE-stained brain lesion tissues in model group,with a large number of inflammatory cells in the interstitium,and extracellular leakage of red blood cells.Compared with sham group,the mNSS and the percentage of water in the brain tissue increased in model group(P＜0.05),the expression levels of IL-6 and IL-1β were also elevated(P＜0.05),and the relative expression levels of p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα,and the rate of apoptosis increased(P＜0.05).Compared with model group,more orderly cellular arrangements were observed in the HE-stained brain tissue in Pterostilbene group,the infiltration of inflammatory cells and red blood cells significantly decreased,and the edema was re-duced.Compared with model group,both the mNSS and the percentage of water in the brain tissue decreased in Pterostilbene group(P＜0.05),the expression levels of IL-6 and IL-1β were also reduced(P＜0.05),the relative expression levels of p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα,and the rate of apoptosis decreased(P＜0.05).Compared with Pterostilbene group,loose and disordered cells were presented in inhibitor group,with a high number of inflammatory cells,red blood cell infiltration,and significant edema.Compared with Pterostilbene group,both the mNSS and the percentage of water in the brain tissue increased in inhibitor group(P＜0.05),the ex-pression levels of IL-6 and IL-1β were elevated(P＜0.05),the relative expression levels of p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα,and the rate of apoptosis increased(P＜0.05).Conclusion Pterostilbene can protect against the secondary injury after cere-bral hemorrhage in rats,and inhibit the neuroinflammatory responses,which may be related to the inhibition of the activation of NF-κB signaling pathway.关键词
紫檀芪/脑出血/继发性脑损伤/炎症反应/NF-κB信号通路/细胞凋亡Key words
Pterostilbene/cerebral hemorrhage/secondary brain injury/inflammatory response/NF-κB signaling pathway/cell apoptosis分类
医药卫生引用本文复制引用
赵桂秋,牟江利,郑晓梅..紫檀芪对脑出血大鼠的脑神经保护作用及其机制[J].山西医科大学学报,2025,56(7):776-782,7.基金项目
四川省医学会科研课题(2018HSD5-10) （2018HSD5-10）
