塔里木大学学报2025，Vol.37Issue(4)：1-10,10.DOI:10.3969/j.issn.1009-0568.2025.04.001
甘草属植物DNA条形码的筛选与应用
Screening and application of DNA barcodes in Glycyrrhiza plants
摘要
Abstract
In this study,we screened the sequences with high nucleic acid variability as candidate DNA barcodes through the plastid genome of Glycyrrhiza,amplified and sequenced them by PCR,and combined with the sequence data from the GenBank database to evaluate the identification ability of different DNA barcodes by phylogenetic tree construction and genetic distance analysis.The results showed that among the 10 candidate DNA barcodes screened,trnF-trnL,trnQ-accD and trnR-trnN had the highest identification success rate,which could separate seven species of the genus Glycyrrhiza,and the minimum interspecific variation was greater than the maximum intraspecific variation in all three sequences and the minimum intraspecific variation was observed.The combination of trnF-trnL,trnQ-accD and trnR-trnN sequences resulted in a 100％identification success rate,which can be used as the optimal sequence combination for accurate identification of Glycyrrhiza.The present study provide a scientific basis for the molecular identification of the genus of Glycyrrhiza.关键词
甘草属/DNA条形码/鉴定/遗传距离Key words
Glycyrrhiza L/DNA barcoding/identification/genetic distance分类
农业科技引用本文复制引用
牛世豪,封鑫鑫,郭馨,刘欣,唐娜,李海文..甘草属植物DNA条形码的筛选与应用[J].塔里木大学学报,2025,37(4):1-10,10.基金项目
塔里木大学大学生创新创业训练计划项目(202210757016) （202210757016）
塔里木大学校长基金硕士人才项目(TDZKSS202254) （TDZKSS202254）
