塔里木大学学报2025，Vol.37Issue(4)：11-16,6.DOI:10.3969/j.issn.1009-0568.2025.04.002
大叶白麻组织培养再生体系的建立
Establishment of tissue culture regeneration system for Poacynum hendersonii
摘要
Abstract
Poacynum hendersonii,which is widely distributed along the Tarim River,has a high value as natural fiber materials.This aim of this study was to establish an efficient callus regeneration system for rapid propagation and orientation breeding.In this study,the different disinfection methods were used to treat the seeds of P.hendersonii so as to select appropriate disinfection methods,and then the stem of P.hendersonii sterile seedlings were used as explants for rapid propagation.By adding different concentrations of plant hormones such as thidiazuron(TDZ),6-Benzylaminopurine(6-BA),naphthylacetic acid(NAA),the optimal hormone ratio for the callus induction,the callus differentiation,and induction of adventitious roots of P.hendersonii was explored.The results showed that the most suitable sterilization method was as follows,first treated with 75％ethanol for 30 seconds,then treated with 1％hydrogen peroxide for 10 min,washed with sterile water 3-4 times,the pollution rate was 0.00％,the germination rate was 61.38％,and the germination was neat.The best medium for callus induction and differentiation was MS+0.5 mg/L TDZ+0.2 mg/L NAA,the induction rate was 90.67％,and the induction rate of adventitious buds was 60.00％;The best medium for rooting was 1/2MS+0.5 mg/L NAA,and the rooting rate could reach 100.00％.This study provides technical support and theoretical reference for the rapid propagation of P.hendersonii,the production of secondary metabolites,and the research of molecular biology.关键词
大叶白麻/愈伤组织/组织培养/植株再生体系Key words
Poacynum hendersonii/callus/tissue culture/plant regeneration system分类
农业科技引用本文复制引用
王祥媛,刘逸泠,刘雪梅,杨熙,郭媛..大叶白麻组织培养再生体系的建立[J].塔里木大学学报,2025,37(4):11-16,6.基金项目
2022年度兵团指导性科技计划项目(2022ZD120) （2022ZD120）
塔里木大学校长基金博士人才项目(TDZKBS202205) （TDZKBS202205）
