中国草地学报2025，Vol.47Issue(8)：1-9,9.DOI:10.16742/j.zgcdxb.20240179
基于转录组测序的紫花苜蓿根系特异性基因筛选
Screening of Root-Specific Genes in Medicago sativa Based on Transcriptome Sequencing
摘要
Abstract
In order to investigate the root-specific genes regulating root growth of alfalfa(Medicago sativa),tran-scriptome sequencing was performed to analyze differentially expressed genes(DEGs)and their functions between the aerial parts(stems and leaves)and root system of alfalfa cultivar Zhongmu No.1.The results showed that compared to aerial parts,a total of 15258 DEGs were identified in root system,including 5095 up-regulated and 10163 down-regulated.The GO functional annotation demonstrated significant enrichment of these DEGs in cellular process,meta-bolic regulation,and biocatalytic activities.KEGG pathway enrichment analysis showed significant enrichment of these DEGs in pathways related to porphyrins and chlorophyll metabolism,photosynthesis-antenna protein synthesis,and carbon fixation in photosynthetic organisms.Six candidate up-regulated genes exhibiting significant differential expres-sion were selected for real-time quantitative PCR validation.The results indicated high concordance between their expression patterns and transcriptome sequencing data.关键词
紫花苜蓿/根系/转录组测序/差异表达基因/实时荧光定量PCRKey words
Medicago sativa/Root system/Transcriptome sequencing/Differentially expressed genes/Real-time PCR分类
农业科技引用本文复制引用
刘晋囡,王晓倩,张驰昊,艾晔,晁跃辉..基于转录组测序的紫花苜蓿根系特异性基因筛选[J].中国草地学报,2025,47(8):1-9,9.基金项目
内蒙古自治区科技重大专项项目专题"抗寒高产苜蓿新品种培育"(2022JBGS00160302) （2022JBGS00160302）
