中国农学通报2025，Vol.41Issue(22)：13-26,14.
秋水仙素对金花菜多倍体的诱导效应
Induction Effect of Colchicine on Polyploidy of Medicago polymorpha
摘要
Abstract
Using the seeds and plantlets of Medicago polymorpha in Shanghai as experimental materials,different concentrations of colchicine were set(0%,0.01%,0.03%,0.05%,0.07%,0.09%,0.11%),and plant tissue culture,plant biochemical physiology,and plant molecular biology techniques were used to investigate the effects of colchicine treatment on the germination of seeds,the growth and development of plantlets,as well as related physiological and biochemical indicators and gene expression in M.polymorpha.The results showed that the addition of colchicine and colchicine soaking to MS medium could inhibit the germination rate of M.polymorpha seeds and the plant height of germinated seedlings,reduce their leaf number,increase their stem thickness,promote their rooting,increase their CAT activity,SOD activity,POD activity,MDA content,soluble sugar content,soluble protein content,and proline content,promote their upregulation of WRKY transcription factor,protein TIFY 8,transcription factor MYB13,NAC transcription factor 47,ABC transporter,late embryogenesis rich protein 2,and ethylene responsive transcription factor ERF110 gene expression.The addition of colchicine to MS medium and soaking in colchicine could inhibit the plant height,leaf number,stem diameter,root number,and root length of plantlets of M.polymorpha,promote their increase of stem diameter,CAT activity,SOD activity,POD activity,MDA content,soluble sugar content,soluble protein content,and proline content of test tube seedlings of M.polymorpha,and promote their upregulation of gene expression of WRKY transcription factor,protein TIFY 8,transcription factor MYB13,NAC transcription factor 47,ABC transporter,late embryogenesis enriched protein 2,and ethylene responsive transcription factor ERF110.The polyploidy induction effect of M.polymorpha seeds on colchicine added medium was lower than the polyploidy induction effect of M.polymorpha seeds soaked in colchicine,the polyploidy induction effect of M.polymorpha seeds soaked in colchicine was lower than the polyploidy induction effect of M.polymorpha plantlets on colchicine added medium,the polyploidy induction effect of M.polymorpha plantlets on colchicine added medium was lower than the polyploidy induction effect of M.polymorpha plantlets soaked in colchicine.The results of this experiment had practical significance for the induction of polyploidy in M.polymorpha,and could provide theoretical support for the study of signal pathways for polyploidy induction in M.polymorpha and the application of colchicine in inducing polyploidy in M.polymorpha.关键词
秋水仙素/金花菜/种子萌发/试管苗/生理生化指标/基因表达Key words
colchicine/Medicago polymorpha/seed germination/plantlet/physiological and biochemical indexes/gene expression分类
农业科技引用本文复制引用
洪森荣,吉莉莉..秋水仙素对金花菜多倍体的诱导效应[J].中国农学通报,2025,41(22):13-26,14.基金项目
国家自然科学基金"江西铅山红芽芋'超低温疗法脱毒'响应机制的研究"(31860084) （31860084）
国家自然科学基金"怀玉山三叶青'超低温疗法脱毒'促进离体根膨大的机理研究"(31960079) （31960079）
国家自然科学基金"江西铅山红芽芋超低温疗法脱毒抑制细胞凋亡抗早衰的机理研究"(32060092) （32060092）
江西省现代农业产业技术体系建设专项"江西牧草种质资源保存与利用"(JXARS-13-赣东站). （JXARS-13-赣东站）
