中国农学通报2025，Vol.41Issue(22)：27-34,8.
海藻多糖-纳米硒复合物的制备与表征
Preparation and Characterization of Alginate Polysaccharide-Selenium Nanoparticles Complex
摘要
Abstract
The study aimed to optimize the preparation process of alginate polysaccharide-selenium nanoparticles(APS-SeNPs)for developing efficient and stable nano-selenium fertilizers.The combination experiment of orthogonal and single-factor was designed to prepare APS-SeNPs.The ratio of absorbance at 410 nm to 490 nm(A410/A490)served as a critical indicator for evaluating particle size and stability of APS-SeNPs.Morphological structure and element distribution characterization of APS-SeNPs were performed using scanning electron microscopy-energy dispersive spectroscopy(SEM-EDS).The research results indicated that the optimal preparation conditions of APS-SeNPs were determined as follows.APS concentration of 1200 mg/L,molar ratio of vitamin C(Vc)to sodium selenite(Na2SeO3)at 4:1,reaction temperature of 45℃,and reaction time of 1.5 h.The prepared APS-SeNPs exhibited uniformly dispersed spherical structure which particle size was(91.30±7.20)nm,with A410/A490 ratio of 1.96±0.01 and selenium(Se)loading capacity of 31.51%.Energy spectrum analysis revealed that the proportion of carbon and oxygen elements in APS-SeNPs increased by 38.97%and 336.94%,respectively,compared with selenium nanoparticles(SeNPs)unmodified by alginate polysaccharide,indicating that alginate polysaccharide modified the surface of selenium nanoparticles effectively.The APS-SeNPs prepared by this optimized process possessed fine stability and Se loading,providing a theoretical basis for the development of novel nano-selenium fertilizers.关键词
纳米硒/海藻多糖/制备/扫描电镜/能谱分析/双波长比色法(A410/A490)/正交试验/单因素试验Key words
selenium nanoparticles/alginate polysaccharide/preparation/scanning electron microscopy/energy spectrum analysis/dual-wavelength spectrophotometry(A410/A490)/orthogonal experimental design/single-factor experiment分类
农业科技引用本文复制引用
靳丹丹,黄正花,昌晓宇,张康康,魏益华,张标金..海藻多糖-纳米硒复合物的制备与表征[J].中国农学通报,2025,41(22):27-34,8.基金项目
赣鄱俊才支持计划-主要学科学术和技术带头人培养项目"水稻中纳米硒转化调控关键技术研究与示范"(20232BCJ22059) （20232BCJ22059）
江西省重点研发计划项目"富硒大米品质提升与标准化生产关键技术研究"(20232BBF60019) （20232BBF60019）
国家自然科学基金项目"OsHSE1调控水稻籽粒硒运输代谢的分子机理"(32260067). （32260067）
