中国农学通报2025，Vol.41Issue(23)：53-63,11.
富硅土壤改良肥料对旺长期植烟土壤微生物群落结构影响
Impact of Silicon-enriched Soil Amendment Fertilizers on Composition of Microbial Community in Tobacco-growing Soils During Vigorous Growing Period
摘要
Abstract
To explore the changes of microbial community structure in tobacco-planting soil after application of silicon-rich soil improvement fertilizer and the effects of functional flora on soil nutrients and yield and quality of flue-cured tobacco,the root soil of flue-cured tobacco variety'K326'was used as the experimental material,and the silicon fertilizer of 75 kg/hm2 was supplemented under the condition of conventional application of organic fertilizer as the base fertilizer,and the local conventional fertilization scheme was used as the control.By recording the agronomic traits of tobacco plants in the field and the economic traits of tobacco leaves,the Illumina Mi Seq high-throughput sequencing was used to measure the fungi,bacteria genes and conventional physical and chemical properties of tobacco root soil in the two treatments.The results showed that the soil available phosphorus,organic matter content and pH,agronomic traits of tobacco plants and economic traits of flue-cured tobacco leaves treated with silicon-rich soil improvement fertilizer were significantly higher than those of the control treatment.There was no significant difference in the classification level of fungi and bacteria between the two treatments.Ascomycota,Basidiomycota,Actinobacteriota,Proteobacteria and Firmicutes were the main fungi and bacteria.At the genus level,Fusarium,Saitozyma,Chaetomium and Sphingomonas were the dominant genera in the silicon-rich soil improvement fertilizer treatment,and unclassified_k__Fungi was the unique genus in this treatment.Among them,Chaetomium,Saitozyma,unclassified_c__Sordariomycetes,Bradyrhizobium and norank_f_JG30-KF-AS9 showed a significant positive correlation with soil nutrients.Effective nitrogen content and pH were greatly affected by fungal microorganisms,and organic matter was greatly affected by bacterial microorganisms.The richness of fungal species in the root soil of tobacco plants treated with silicon-rich soil improvement fertilizer was lower than that of the control treatment,and saprotroph fungi were the main fungal types.The bacterial species richness and KO number were significantly higher than those of the control treatment.The results showed that the application of silicon-rich soil improved fertilizer could not only increase the yield and output value of flue-cured tobacco,but also supplement soil fertility and regulate soil acidity,and slow down the acidification degree of sandy soil.In this study,the effects of soil improvement fertilizer on acidified sandy soil in high altitude area were compared from the perspective of soil microbial structure and function prediction,in order to provide scientific reference for the scientific and reasonable application of agricultural production fertilizer and the production of microbial fertilizer in similar areas.关键词
Illumina Mi Seq/土壤改良/微生物结构/功能预测Key words
Illumina Mi Seq/soil enhancement/microbial composition/prediction of functions分类
农业科技引用本文复制引用
魏康凯,陈厚任,张泽林,孔广超,李兴红,高庭猛,黄飞燕,农晚意..富硅土壤改良肥料对旺长期植烟土壤微生物群落结构影响[J].中国农学通报,2025,41(23):53-63,11.基金项目
中国烟草总公司重庆中烟工业有限责任公司项目"重庆中烟双江烟叶基地关键生产技术优化及应用"(2022533517000895) （2022533517000895）
云南省科技厅基础研究专项面上项目"MicroRNA调控烟草响应黑胫病菌胁迫的分子机制研究"(202401AT070034). （202401AT070034）
