中国中西医结合儿科学2025，Vol.17Issue(4)：292-297,后插1,7.DOI:10.20274/j.cnki.issn.2025.04.004
基于神经元凋亡机制探究平痫冲剂及其拆方的抗痫作用机制
Exploring the anti-epileptic mechanism of Pingxian granules and its split formula based on neuronal apoptosis
摘要
Abstract
Objective To observe the anti-epileptic effects of Pingxian granules and its split formula,and explore their anti-epileptic mechanisms.Methods Totally 270 4-week-old Wistar rats were randomly divided into a blank control group of 36 rats and a model group of 234 rats.The epilepsy model was prepared by intraperitoneal injection of pentylenetetrazole.After the successful preparation of epilepsy model rats(n=216),they were randomly divided into a model control group,a positive control group,a phlegm-resolving group,a blood stasis-removing group,wind-stopping group,and a Pingxian group,with 36 rats in each group.The positive control group was given 0.36 g/kg of sodium valproate(VPA),while the phlegm-resolving group,blood stasis-removing group,and wind-stopping group were all given medications from the split formula of Pingxian granules.The phlegm-resolving group was given 1.40 g/kg of phlegm-resolving drugs(Acorus tatarinowii and Dannanxing)by gavage;the blood stasis-removing group was given 2.08 g/kg of phlegm-resolving drugs(Danshen and Yujin)by gavage;the wind-stopping group was given 2.96 g/kg of wind-stopping drugs(Bombyx mori,Tianma,Daizheshi and Baishao)by gavage;the Pingxian group was given 3.22 g/kg of anti-epileptic drugs(composed of the aforementioned phlegm-resolving drugs,blood stasis-removing drugs,and wind-stopping drugs)by gavage.The blank control group and model control group were given an equal amount of physiological saline by gavage.Each group was treated by gavage for 21 days.Results Compared with the model control group,the Pingxian group and each group with split formula showed reduced positive expression of Bax protein at 24 h,48 h,72 h,7 d,14 d,and 21 d after drug intervention(P<0.05).Compared with the model control group,the Pingxian group and each group with split formula showed enhanced positive expression of Bcl-2 protein at 48 h,72 h,7 d,14 d,and 21 d after drug intervention(P<0.05).Compared with the model control group,the positive control group,phlegm-resolving group,blood stasis-removing group,wind-stopping group and Pingxian group showed varying degrees of repair in the number and morphology of pyramidal neurons,with the Pingxian group showing greater improvement than the other groups with split formula,while the blood stasis-removing group showed no significant changes.Conclusion Pingxian granules and its split formula may exert anti-epileptic effects by inhibiting neuronal apoptosis,downregulating Bax protein expression,and upregulating Bcl-2 protein expression.关键词
癫痫/神经元凋亡/平痫冲剂/机制研究/儿童Key words
Epilepsy/Neuronal apoptosis/Pingxian granules/Mechanism research/Child分类
医药卫生引用本文复制引用
高旅,刘驰,姚政宇,景一珏,吴丽萍,史正刚..基于神经元凋亡机制探究平痫冲剂及其拆方的抗痫作用机制[J].中国中西医结合儿科学,2025,17(4):292-297,后插1,7.基金项目
国家自然科学基金项目(81460739,82305318) National Natural Science Foundation of China Project(No.81460739,82305318) （81460739,82305318）
