中国当代医药2025，Vol.32Issue(22)：9-14,6.DOI:10.3969/j.issn.1674-4721.2025.22.02
骆驼蓬碱联合紫杉醇在体外和体内对人胃癌细胞SGC-7901细胞增殖的影响及其机制
Effect and mechanism of harmine combined with Paclitaxel on the prolif-eration of human gastric cancer cell SGC-7901 in vitro and in vivo
孙坤 1张皓 2吴洪文1
作者信息
- 1. 山东省公共卫生临床中心消化内科,山东济南 250000
- 2. 南京医科大学第一附属医院肿瘤内科,江苏南京 210000
- 折叠
摘要
Abstract
Objective To explore the effect of harmine and Paclitaxel on cell proliferation in human gastric cancer cell SGC-7901 in vitro and in vivo.Methods The inhibitory effect of harmine and Paclitaxel on the growth of gastric cancer cells were detected by the MTT method.Western blot was used to analyze the effect of harmine and Paclitaxel on the expression levels of cyclooxygenase-2(COX-2),proliferating cell nuclear antigen(PCNA),B-cell lymphoblastoma-2(Bcl-2),and Bcl-2 associated X protein(Bax).The inhibitory effect of harmine and Paclitaxel on the proliferation of gastric cancer cells in vivo were detected in male nude mice without thymus.Results Harmine and Paclitaxel inhibited cell proliferation in a dose-dependent manner.When harmine was combined with Paclitaxel,it can down-regulate the expressions of COX-2,PCNA and Bcl-2,increase the expression level of Bax,and exert a synergistic inhibitory effect on the proliferation of SGC-7901 cells both in vivo and in vitro.Conclusion Harmine and Paclitaxel can exert anti-tumor effects in the treatment of gastric cancer.Compared with the use alone,the combined use of the two drugs can more effectively inhibit the development of gastric cancer,and its mechanism of action may be achieved by down-regulating the expression level of COX-2.关键词
骆驼蓬碱/紫杉醇/胃癌/环氧化酶-2Key words
Harmine/Paclitaxel/Gastric cancer/Cyclooxygenase-2分类
医药卫生引用本文复制引用
孙坤,张皓,吴洪文..骆驼蓬碱联合紫杉醇在体外和体内对人胃癌细胞SGC-7901细胞增殖的影响及其机制[J].中国当代医药,2025,32(22):9-14,6.
