农业现代化研究2025，Vol.46Issue(4)：777-788,12.DOI:10.13872/j.1000-0275.2025.0281
重组固氮菌提升食叶草耐盐碱性能及其氮肥替代潜力
Inoculation of Pseudomonas fluorescens increases of salt alkali resistance for edible dock and its potential for nitrogen fertilizer substitution
摘要
Abstract
Highly saline-alkaline soil environments inhibit crop growth and development and are a key bottleneck when attempting to improve and utilize saline-alkaline land.To address the critical challenge of saline-alkaline soil remediation,this study investigated the efficacy of recombinant nitrogen-fixing(nif)bacteria(Pseudomonas fluorescens CHA0-nif)in enhancing seed germination of an edible dock(Rumex spp.)under saline-alkaline stress and their potential to replace chemical nitrogen fertilizers.Indoor germination assays and pot experiments with graded nitrogen reduction(0%,10%,20%,30%)were conducted.Key findings include:1)Under moderate saline stress,CHA0-nif significantly improved germination rates germination rates by 13.26%～16.40%and shoot elongation by 8.89%～34.56%,outperforming the wild-type strain(WT).No growth promotion was observed under high stress.2)Soil nitrate nitrogen content increased by 27.25%～54.59%with CHA0-nif inoculation,with sustained enhancement under 10%～20%nitrogen reduction.3)CHA0-nif increased root biomass by 48.02%and total plant nitrogen accumulation by 41.37%.Stable aboveground biomass and nitrogen uptake were maintained at 20%nitrogen reduction,whereas 30%reduction caused significant declines in all parameters.These results demonstrate that CHA0-nif enhances forage grass adaptation to moderate saline-alkaline stress and enables 20%nitrogen fertilizer replacement,offering a novel microbial strategy for sustainable saline soil rehabilitation.关键词
固氮菌/基因编辑/盐碱胁迫/食叶草/氮肥替代Key words
nitrogen-fixing bacteria/gene editing/salinity stress/edible dock/nitrogen fertiliser replacement分类
农业科技引用本文复制引用
姚连春,甘琪林,郑伟劲,涂强,朱光旭,张友明,陈香碧..重组固氮菌提升食叶草耐盐碱性能及其氮肥替代潜力[J].农业现代化研究,2025,46(4):777-788,12.基金项目
微生物改造技术全国重点实验室开放课题基金项目(M2022-05) （M2022-05）
山东省重点研发计划项目(2022SFGC0304) （2022SFGC0304）
深圳市科技计划平台和载体专项筹建启动项目(ZDSYS20220303153551001) （ZDSYS20220303153551001）
喀什大学研究生科研创新项目(KD2023KY051).Supported by the State Key Laboratory of Microatory Technology Open Projects Fund(M2022-05) （KD2023KY051）
Key Research and Development Program of Shandong Province(2022SFGC0304) （2022SFGC0304）
Science and Technology Planning Platform and Carrier Special Preparation and Startup Project of Shenzhen(ZDSYS20220303153551001) （ZDSYS20220303153551001）
Kashi University Postgraduate Research and Innovation Program(KD2023KY051). （KD2023KY051）
