天津科技大学学报2025，Vol.40Issue(4)：33-41,58,10.DOI:10.13364/j.issn.1672-6510.20240053
海洋草生链霉菌BAS-28次级代谢产物与生物合成途径的分析鉴定
Secondary Metabolites and Underlying Biosynthetic Pathways of Streptomyces pratensis BAS-28 from Marine Sources
摘要
Abstract
In this study,a strain originating from the South China Sea was selected.Through morphological and molecular biology techniques,the species was first identified,and the strategy of one strain many compounds(OSMAC)was then em-ployed to activate silent gene clusters.Various chromatographic separation techniques were further used to isolate com-pounds,and their structures were determined by comparing spectral data with literature.Finally,the biosynthetic gene clus-ters were analyzed using antiSMASH to deduce the biosynthetic pathways.Phylogenetic analysis of the 16S rDNA gene sequence identified the strain as Streptomyces pratensis.Three monomeric compounds,namely mirubactin B(1),mirubactin C(2),and benadrostin(3)were isolated from the fermentation crude extract.The gene clusters encoding the synthesis of these compounds were successfully identified.Compounds 1 and 2 belong to non-ribosomal peptide synthase(NRPS)siderophores,playing an important role in the survival and growth of microorganisms.Compound 3 is an inhibitor of poly(ADP-ribose)synthetase,and its activity is related to the pathogenesis of various diseases.Through the analysis of the bio-synthetic pathway processes of compounds 1 to 3,this study has provided important reference bases for drug development.关键词
次级代谢产物/链霉菌/OSMAC策略/生物合成基因簇Key words
secondary metabolites/Streptomyces/OSMAC strategy/biosynthetic gene clusters分类
医药卫生引用本文复制引用
刘竹青,王俊潇,徐飞,罗学刚..海洋草生链霉菌BAS-28次级代谢产物与生物合成途径的分析鉴定[J].天津科技大学学报,2025,40(4):33-41,58,10.基金项目
国家重点研发计划项目(2021YFC2100600) （2021YFC2100600）
