天津科技大学学报2025，Vol.40Issue(4)：51-58,8.DOI:10.13364/j.issn.1672-6510.20230236
基于全自动生化分析仪检测(1,3)-β-D-葡聚糖的性能评估
Performance Evaluation of a(1,3)-β-D-Glucan Detection Method Using an Automatic Biochemical Analyser
摘要
Abstract
Serum(1,3)-β-D-glucan assay is an important indicator for the diagnosis of invasive fungal infections(IFI).In this study,a fully automated biochemical analyzer and corresponding reagents were developed to quantify the concentration of(1,3)-β-D-glucan by dynamically monitoring the rate of change in the absorbance of p-nitroaniline(pNA)in solution,based on an enzymatic cascade reaction in the reagents triggered by(1,3)-β-D-glucan to produce free p-nitroaniline(pNA)causing absorbance changes.The small size of the instrument makes it easy to carry and operate.It can be used to perform prompt on-site testing(POCT)without the need for manual operation,which can simplify the processes of sample addition,mixing and result processing,while improve the sensitivity and accuracy of the(1,3)-β-D-glucan detection.By testing serum or plasma samples from 165 male and 135 female patients,the method was found to have a wide linear range(31.25-1000 pg/mL),a low blank limit(14.17 pg/mL)and a detection limit(25.78 pg/mL),and the results of the ac-curacy and precision tests met the expected requirements,and the results of the interference experiments proved the method's stability and reliability.The analyzer and reagent combination exhibits high efficiency,accuracy,and rapid detection cap-abilities,making it a valuable tool for the early and rapid diagnosis of invasive fungal infections.关键词
(1,3)-β-D-葡聚糖/即时检验/全自动生化分析仪/侵袭性真菌感染/早期快速诊断Key words
(1,3)-β-D-glucan/point of care testing/automatic biochemical analyzer/invasive fungal infections/early and rapid diagnosis分类
医药卫生引用本文复制引用
武雅媚,张玉,翟栓柱,钱芃橙,刘春龙,钟成..基于全自动生化分析仪检测(1,3)-β-D-葡聚糖的性能评估[J].天津科技大学学报,2025,40(4):51-58,8.基金项目
天津市科委基金项目(22ZYQYSY00040) （22ZYQYSY00040）
