武警医学2025，Vol.36Issue(8)：658-663,6.
抗原修复条件对Pax-8和CD10免疫组织化学染色效果的影响
Effect of antigen retrieval conditions on the immunohistochemical staining results of Pax-8 and CD10
艾亚昆 1苏荣 2刘静 1任飞垚 1温晶 1陈迪 1郭爱桃1
作者信息
- 1. 100039 北京,解放军总医院第三医学中心病理科
- 2. 100039 北京,解放军总医院第三医学中心病理科||046000,长治医学院基础医学部
- 折叠
摘要
Abstract
Objective To investigate the effect of antigen retrieval time and pH value of the retrieval solution on the immuno-histochemical staining results of Pax-8 and CD10.Methods Fifteen surgical specimens of kidneys and livers(15 cases respec-tively)were selected,and the EnVision two-step immunohistochemical method was used to perform antigen retrieval with pH8.0 and pH9.0 in renal tissue Pax-8 and liver tissue CD10,respectively.The retrieval time was 1.5 min,2 min,2.5 min,and 3 min.The staining was evaluated by the main pathologist in a blind review.Results The positive expression of Pax-8 and CD 10 in both groups of retrieval solutions was good(P＜0.05).The double-factor analysis of variance of renal tissue Pax-8 antibody staining showed that the main effect of retrieval solution pH was significant(P＜0.01,partial η2=0.424).Background staining in pH 8.0 retrieval solu-tion groups of 1.5 min,2 min,2.5 min,and 3 min was significantly lighter than that in pH 9.0 groups,with statistically significant difference(P＜0.01).There was no statistically significant difference in the interaction effect of the retrieval time and the interaction effect between the two factors.For hepatic tissue CD10 antibody staining,double-factor ANOVA indicated a significant main effect of pH(P＜0.01,partial η2=0.076),non-significant main effect of retrieval r time(P＞0.05),but significant interaction effect(P＜0.05,partial η2=0.068).The pH 8.0-1.5 min group showed significantly lighter background staining than pH 9.0-1.5 min group(P＜0.01).Within pH 8.0 groups,no significant differences were observed across time points(P＞0.05).Under pH 9.0 condi-tions,background staining progressively decreased with extended repair time,reaching the lightest level in 3-min group.Conclu-sions The background of Pax-8 staining n renal tissue is not related to antigen retrieval time,while pH 8.0 retrieval solution can re-duce the background staining.For hepatic tissue CD 10 staining,antigen retrieval duration should be appropriately adjusted according to pH variation of retrieval solutions:1.5 min for pH 8.0 and 3 min for pH 9.0.关键词
免疫组化/抗原修复/pH/背景着色Key words
immunohistochemistry/antigen retrieval/pH/background coloration分类
医药卫生引用本文复制引用
艾亚昆,苏荣,刘静,任飞垚,温晶,陈迪,郭爱桃..抗原修复条件对Pax-8和CD10免疫组织化学染色效果的影响[J].武警医学,2025,36(8):658-663,6.
