诊断学理论与实践2025，Vol.24Issue(4)：423-430,8.DOI:10.16150/j.1671-2870.2025.04.009
血清Hsp90α、eotaxin-2、TRAF6联合检测对早期结肠直肠癌的诊断及预测预后价值
Diagnostic and prognostic value of combined detection of serum Hsp90α,eotaxin-2,and TRAF6 in early colorec-tal cancer
摘要
Abstract
Objective To analyze the expression and clinical diagnostic value of serum heat shock protein 90α(Hsp90α),eosinophil chemotactic protein 2(eotaxin-2),and tumor necrosis factor receptor associated factor 6(TRAF6)in patients with early colorectal cancer.Methods From May 2019 to December 2020,119 patients with stage Ⅰ and stage Ⅱcolorectal cancer diagnosed by postoperative pathology admitted to our hospital were consecutively included in the colorec-tal cancer group.After a 3-year follow-up,surviving patients were divided into a recurrence and metastasis group and a non-recurrence and metastasis group.Additionally,121 patients with colorectal adenoma were collected as the benign lesion group.Enzyme-linked immunosorbent assay(ELISA)method was used to detect serum levels of Hsp90α,eotaxin-2,and TRAF6.If the level was higher than the median expression level among all patients,it was defined as high expression;oth-erwise,it was defined as low expression.Receiver operator characteristic(ROC)curve was used to analyze the diagnostic value of serum Hsp90α,eotaxin-2,and TRAF6 in distinguishing early colorectal cancer from benign lesions and in predic-ting recurrence and metastasis in colorectal cancer patients.Thearea under the curve(AUC)was comparedusing Z-test.Ka-plan-Meier method was used to analyze the relationship between these indicators and 3-year survival rate of colorectal can-cer patients.Results The serum levels of Hsp90α,eotaxin-2,and TRAF6 in the colorectal cancer group were higher than those in the benign lesion group(P<0.05).In colorectal cancer patients with a tumor diameter of≥5 cm and TNM stage Ⅱ,the serum levels of Hsp90α,eotaxin-2,and TRAF6 were higher than in those with a tumor diameter of<5 cm and TNM stage Ⅰ(P<0.05).The AUC for differentiating early colorectal cancer from benign lesions using serum Hsp90α,eotaxin-2,and TRAF6 alone and in combination were 0.783(95%CI:0.723-0.843),0.795(95%CI:0.739-0.851),0.753(95%CI:0.690-0.816),and 0.906(95%CI:0.867-0.945),respectively.The combined AUC was higher than the individual AUC of each indicator(Z=3.411,3.151,4.054,P<0.05),with sensitivity values of 52.99%,85.73%,73.12%,and 86.68%,and specificity of 75.98%,59.52%,71.12%,and 82.62%,respectively.The diagnostic thresholdsfor Hsp90α,eotaxin-2,and TRAF6 were 178.27 ng/mL,198.77 pg/mL,and 3.49 μg/mL,respectively.A total of 119 colorectal cancer patients were fol-lowed up for 3 years,with 92 surviving(including 14 survivors withrecurrence and metastasis)and 27 dying due to recur-rence and metastasis.The 3-year survival rate of patients with high serum expression of Hsp90α,eotaxin-2,and TRAF6 were 62.07%,61.67%and 65.57%,which were lower than that of patients with low expression,which were 91.80%,93.22%and 89.66%(P<0.05).The serum levels of Hsp90α,eotaxin-2,and TRAF6 in colorectal cancer patients with recur-rence and metastasis were higher than those in the non-recurrence and metastasis group(P<0.05).The AUC for predicting colorectal cancer recurrence and metastasis using serum Hsp90 α,eotaxin-2,and TRAF6 alone and in combination were 0.836(95%CI:0.760-0.911),0.701(95%CI:0.597-0.804),0.821(95%CI:0.741-0.900),and 0.968(95%CI:0.934-0.998),respectively.The combined AUC was higher than the individual AUC of each indicator alone(Z=3.073,4.770,3.351,P<0.05).Conclusion The serum levels of Hsp90α,eotaxin-2,and TRAF6 are higher in patients with early colorec-tal cancer,and they are associated with the patient's prognosis.The combination of these three is expected to serve as clini-cal auxiliary diagnostic and prognostic markers.关键词
早期结肠直肠癌/热休克蛋白90α/噬酸性细胞趋化因子2/肿瘤坏死因子受体相关因子6/诊断Key words
Early colorectal cancer/Heat shock protein 90α/Eosinophil chemotactic protein 2/Tumor necrosis factor receptor associated factor 6/Diagnosis分类
医药卫生引用本文复制引用
江南,许亚聪,刘佳瑶,孙荣,郑高歌,徐菱遥,闫春晓..血清Hsp90α、eotaxin-2、TRAF6联合检测对早期结肠直肠癌的诊断及预测预后价值[J].诊断学理论与实践,2025,24(4):423-430,8.基金项目
开封市科技发展计划项目(2303015) （2303015）
