中国口腔种植学杂志2025，Vol.30Issue(4)：370-376,7.DOI:10.12337/zgkqzzxzz.2025.08.007
糖尿病患者与健康人A-PRF中成骨与炎症因子的对比研究
Comparative study on the release of osteogenic and inflammatory factors in advanced platelet-rich fibrin between diabetic and healthy populations
摘要
Abstract
Objective To compare the release kinetics of key osteogenic growth factors and inflammatory cytokines in advanced platelet-rich fibrin(A-PRF)between patients with diabetes mellitus(DM)and healthy populations.Methods Participants meeting the inclusion and exclusion criteria were assigned to either the DM group(n=8)or the healthy control group(n=8).Venous blood was centrifuged to prepare A-PRF membranes,and eluates were collected at 15 min,60 min,8 h,1 d,3 d,10 d,14 d,and 21 d post-incubation.The concentrations of platelet-derived growth factor-BB(PDGF-BB),vascular endothelial growth factor(VEGF),transforming growth factor-beta(TGF-β),epidermal growth factor(EGF),tumor necrosis factor-alpha(TNF-α),and interleukin-6(IL-6)were quantified using enzyme-linked immunosorbent assay(ELISA)and analyzed comparatively.Results(1)Cumulative PDGF-BB release from A-PRF in the DM group was consistently lower than that in healthy controls,with statistically significant differences observed from 15 min onward(P<0.01).(2)VEGF release from A-PRF was significantly reduced in the DM group from day 1 to day 21 compared to controls(P<0.01).(3)No statistically significant difference in cumulative EGF release was observed between the two groups(P>0.05).(4)Cumulative TGF-β release from A-PRF was significantly lower in the DM group from day 1 onward(P<0.05).(5)TNF-α release from A-PRF was significantly higher in the DM group than in healthy controls from 8 h onward(P<0.05).(6)No statistically significant difference in cumulative IL-6 release was observed between the two groups(P>0.05).Conclusion Compared to healthy individuals,DM patients exhibited significantly lower cumulative release of osteogenic growth factors(PDGF-BB,VEGF,TGF-β)from A-PRF and elevated levels of the inflammatory cytokine TNF-α.No significant differences were observed in EGF and IL-6 release.关键词
改良富血小板纤维蛋白/糖尿病/生长因子/炎症因子/骨愈合Key words
Advanced platelet-rich fibrin(A-PRF)/Diabetes mellitus/Growth factors/Inflammatory factors/Bone healing引用本文复制引用
农英娇,黎永薇,陆婵,邓翰林,唐佳勤,李淑洁,梁丽华..糖尿病患者与健康人A-PRF中成骨与炎症因子的对比研究[J].中国口腔种植学杂志,2025,30(4):370-376,7.基金项目
2023年广西高校中青年教师科研基础能力提升项目(2023KY0503) 2023 Basic Research Capacity Enhancement Project for Young and Middle-Aged Teachers in Guangxi Higher Education Institutions(2023KY0503) （2023KY0503）
