中国生物防治学报2025，Vol.41Issue(4)：808-818,11.DOI:10.16409/j.cnki.2095-039x.2024.11.010
通过弱化黄粉虫幼虫体壁提升摩泽蒲螨繁育效率
Improvement of Breeding Efficiency of Pyemotes moseri by Weakening the Integument of Tenebrio molitor
摘要
Abstract
Pyemotes moseri is an ectoparasite mite of insects,showing a great potential in biological control of boring pests.In this study,RNAi technology was applied to weaken larval integument of Tenebrio molitor to improve the efficiency of breeding P.moseri by mealworm.The main results showed that the prokaryotic expression plasmid of PPO gene was successfully constructed to induce the expression of dsTmPPO,which verified that dsTmPPO injection effectively reduced the relative expression level of PPO gene with an interference efficiency of 95.16％,resulting in an effective reduction of integument thickness.The integument thickness of the head,thorax and abdomen of mealworm was reduced by 20.9％,23.5％and 37.5％,respectively,compared to the dsEGFP control group.The parasitism efficiency of P.moseri measured after feeding the mealworm with dsTmPPO showed that there were 221 physogastric females of P.moseri in the dsTmPPO treatment group,significantly higher than that in the dsEGFP control group(115 physogastric females).In conclusion,feeding dsTmPPO to mealworm can weaken the integument and improve the breeding efficiency of P.moseri effectively.关键词
摩泽蒲螨/黄粉虫/RNAi/体壁弱化/繁育效率Key words
Pyemotes moseri/Tenebrio molitor/RNAi/integument softening/breeding efficiency分类
农业科技引用本文复制引用
史可桂,苏晓琪,王敦..通过弱化黄粉虫幼虫体壁提升摩泽蒲螨繁育效率[J].中国生物防治学报,2025,41(4):808-818,11.基金项目
国家重点研发计划(2022YFD1401001) （2022YFD1401001）
西北农林科技大学秦岭研究院科技创新专项(2452023301). （2452023301）
