中国生物防治学报2025，Vol.41Issue(4)：862-876,15.DOI:10.16409/j.cnki.2095-039x.2025.02.051
藜麦茎基腐病拮抗菌LMNS-M9的发酵条件优化及可湿性粉剂研制
Optimization of Fermentation Conditions of Antagonistic Fungus LMNS-M9 against Quinoa Basal Stem Rot and Development of Wettable Powder
摘要
Abstract
In order to develop a biocontrol agent for the prevention and control of quinoa basal stem rot,this study utilized Trichoderma afroharzianum LMNS-M9 as the primary research object.Its antifungal activity was determined,and then the single-factor and response surface tests were designed and performed to optimize the fermentation condition with the inhibition rate as the indexes.On this basis,the carriers and additives were screened to make wettable powder,and then the formula was optimized and the quality was tested.The results showed that strain LMNS-M9 possess high and broad-spectrum antifungal activity,and the inhibition rate against Fusarium solani reached 69.59％.The optimal fermentation conditions of strain LMNS-M9 were as follows:liquid volume 118.84 mL/250 mL,inoculum 0.51％,fermentation time 114.24 h,and rotation speed 210 r/min.After optimization,the inhibition rate of the spore solution of strain LMNS-M9 against F solani was 86.98％,which was 125.00％higher than that before optimization.The composition of LMNS-M9 wettable powder obtained by adjuvant screening and formulation optimization is as follows:spore powder 20％,diatomite 67％,polyethylene glycol 3％,NNO 7％,dextrin 3％,spore content was 3.08× 109 CFU/g,all indicators meet the national standards.The results can provide a theoretical basis for the green control of quinoa basal stem rot and the industrial production of LMNS-M9 wettable powder.关键词
非洲哈茨木霉/茄病镰孢菌/发酵条件/可湿性粉剂Key words
Trichoderma afroharzianum/Fusarium solani/fermentation conditions/wettable powder分类
农业科技引用本文复制引用
张文静,徐紫璐,吕红,秦楠,殷辉,赵晓军,任璐..藜麦茎基腐病拮抗菌LMNS-M9的发酵条件优化及可湿性粉剂研制[J].中国生物防治学报,2025,41(4):862-876,15.基金项目
山西省重点研发计划项目(2022ZDYF117) （2022ZDYF117）
"特""优"农业高质量发展科技支撑工程项目(TYGC25-01,TYGC25-02) （TYGC25-01,TYGC25-02）
山西省研究生教育创新计划支持项目(2024SJ134) （2024SJ134）
