中国药房2025，Vol.36Issue(17)：2106-2112,7.DOI:10.6039/j.issn.1001-0408.2025.17.05
基于富勒醇-氧化铈复合体系的长效光防护纳米凝胶的制备与评价
Preparation and evaluation of long-acting light-protective nanogel based on fullerenol-cerium oxide composite system
摘要
Abstract
OBJECTIVE To develop a long-acting light-protective nanogel with both physical barrier and chemical clearance functions,and evaluate its performance.METHODS The photoprotective nanogel composed of mussel mucin and sodium hyaluronate was constructed based on a fullerenol-cerium oxide composite nano system,namely fullerenol-cerium oxide nanogel(FCN),and was characterized.The antioxidant capacity of FCN was evaluated using in vitro free radical scavenging experiments;its UV shielding ability was assessed by using an SPF value detector;its biosafety was assessed according to the requirements of the Guidelines for Drug Safety Evaluation;skin adhesion was assessed using small animal 3D live imaging technology;its sun protection ability was assessed through skin sunscreen detection and histopathological observation.RESULTS The average particle sizes of cerium oxide and fullerenol nanoparticles in FCN were about 20 and 10 nm,respectively,and FCN exhibited good UV absorption and free radical scavenging abilities.SPF value of FCN was 58.95±0.82,and the ultraviolet A protection level value was 6.21±0.15.No pathogenic colonies such as Staphylococcus aureus,were detected in the nanogel,and the contents of lead,arsenic,mercury and cadmium all met the standards for pharmaceutical excipients;FCN group did not show any irritating reactions such as erythema,edema,or desquamation;blood biochemical indicators of the FCN group were within the normal reference range.The material clearance rate of mice in the artificial sweat flushing group was less than 30%,while the material clearance rate of mice in the dry cleaning group reached about 92%.The mice in the protective group did not show obvious erythema or ulcer formation throughout the experiment.Histopathology showed that the fibers were arranged in an orderly manner,and the number of collagen fibers was close to that of the control group.CONCLUSIONS The FCN formulation constructed in this study meets the relevant requirements of the Chinese Pharmacopoeia,has good safety and skin compatibility,and achieves dual synergistic protection of UV shielding and free radical scavenging.关键词
纳米凝胶/富勒醇/氧化铈/高原紫外线损伤/抗氧化/光防护Key words
nanogel/fullerenol/cerium oxide/high altitude ultraviolet damage/antioxidant/light protection分类
医药卫生引用本文复制引用
张天龙,刘佳,赵庆,周玥,杨明,罗倩钰..基于富勒醇-氧化铈复合体系的长效光防护纳米凝胶的制备与评价[J].中国药房,2025,36(17):2106-2112,7.基金项目
国家自然科学基金项目(No.82102448) （No.82102448）
四川省医学科技创新研究会项目(No.YCK-KY-YCZD2024-104) （No.YCK-KY-YCZD2024-104）
