中国药房2025，Vol.36Issue(17)：2120-2126,7.DOI:10.6039/j.issn.1001-0408.2025.17.07
超氧化物歧化酶抑制AFP表达对肝癌细胞PLC/PRF/5恶性生物学行为的影响
Effects of superoxide dismutase inhibition of AFP expression on the malignant biological behavior of PLC/PRF/5 liver cancer cells
摘要
Abstract
OBJECTIVE To explore the effect of superoxide dismutase(SOD)administration on the malignant behavior of PLC/PRF/5 liver cancer cells,and analyze the correlation between SOD and alpha-fetoprotein(AFP)expression,to provide new ideas for targeting AFP with SOD as a drug for hepatocellular carcinoma.METHODS Normal human liver cells L-02,AFP-negative human liver cancer cells HLE,and AFP-positive human liver cancer cells PLC/PRF/5 were used as experimental cells.Western blot assay and SOD activity detection kit were used to detect the expression of AFP,SOD and activity of SOD in cells before and after changing AFP expression;the effects of different concentrations of SOD[0(control),0.188,0.375,0.75,1.5,3 U/mL]administration on the migration and proliferation of PLC/PRF/5 cells were detected using cell scratch assay and CCK-8 assay.The effects of SOD overexpression on the expression of malignant biological behavior-related proteins AFP and sarcoma virus protein(Src)in PLC/PRF/5 cells were detected using Western blot.RESULTS Compared with L-02 group and HLE group,the expression levels of SOD1 and SOD2,and SOD activity in PLC/PRF/5 cells were significantly reduced(P＜0.05).After down-regulating AFP expression in PLC/PRF/5 cells,compared with PLC/PRF/5 group,the expression levels of SOD1 and SOD2,as well as SOD activity,were significantly increased in the PLC/PRF/5-shAFP group(low-expression)(P＜0.05).After 48 hours of SOD treatment,compared with control group,the scratch healing rates of PLC/PRF/5 cells in the 0.375,0.75,1.5 and 3 U/mL SOD groups were significantly reduced(P＜0.05);after 72 hours of SOD treatment,compared with control group,the scratch healing rates of PLC/PRF/5 cells in the 0.375,0.75,and 1.5 U/mL SOD groups were significantly reduced(P＜0.05 or P＜0.01).Compared with control group,proliferation rates of PLC/PRF/5 cells were significantly reduced in the 0.375,0.75,1.5 and 3 U/mL SOD groups(P＜0.05 or P＜0.01).Compared with the PLC/PRF/5 group before up-regulating SOD1 and SOD2 expression,the expression levels of AFP and Src in the PLC/PRF/5-oeSOD1 and PLC/PRF/5-oeSOD2 groups(over-expression)after up-regulating SOD1 and SOD2 expression were significantly reduced(P＜0.05).CONCLUSIONS A certain concentration of SOD can inhibit malignant behavior such as migration and proliferation of PLC/PRF/5 cells,and the expression level and activity of SOD are negatively correlated with AFP.关键词
超氧化物歧化酶/甲胎蛋白/肝细胞癌/恶性生物学行为/抗癌药物Key words
superoxide dismutase/alpha fetoprotein/hepatocellular carcinoma/malignant behavior/anti-cancer drugs分类
医药卫生引用本文复制引用
陈栘,陈宝莹,周育丽,徐海霞,曹钰,谷悦,朱明月,李孟森..超氧化物歧化酶抑制AFP表达对肝癌细胞PLC/PRF/5恶性生物学行为的影响[J].中国药房,2025,36(17):2120-2126,7.基金项目
国家自然科学基金-地区科学基金项目(No.82460602) （No.82460602）
海南省自然科学基金项目(No.822RC710) （No.822RC710）
海南省科协青年科技英才学术创新计划项目(No.QCXM201922) （No.QCXM201922）
海南医科大学大学生创新创业训练计划项目(No.X202411810104) （No.X202411810104）
