中国药房2025，Vol.36Issue(17)：2127-2133,7.DOI:10.6039/j.issn.1001-0408.2025.17.08
生脉散对气阴两虚型心力衰竭小鼠的改善作用及机制研究
Improvement effect and mechanism of Shengmai powder on heart failure mice with qi-yin deficiency
摘要
Abstract
OBJECTIVE To study the improvement effect and mechanism of Shengmai powder on heart failure(HF)mice with qi-yin deficiency.METHODS The mice were randomly divided into blank group(water),model group(water),Shengmai powder low-,medium-,and high-dose groups[2.61,5.22 and 10.44 g/kg(based on crude drug dosage)]and positive control group(metoprolol,30 mg/kg),with 10 mice in each group.Except for the blank group,all other groups were subcutaneously injected with D-galactose,and a qi-yin deficiency HF mice model was established by continuous food restriction and weight-bearing swimming.At the same time of modeling,the corresponding medicine/water was gavaged once a day for five weeks.The general state of mice was recorded and the traditional Chinese medicine(TCM)syndrome score was evaluated.Behavioral experiments were conducted to investigate the total distance of open field action,the percentage of immobility time,and the swimming exhaustion time of mice.The contents of aspartate transaminase(AST),creatine kinase(CK)and lactate dehydrogenase(LDH)in the serum of mice were detected;cardiac function indexes[heart rate,left ventricular ejection fraction(LVEF),left ventricular end systolic diameter(LVESD),left ventricular end diastolic diameter(LVEDD),left ventricular mass index and whole heart mass index]were all detected;the histopathological morphology of mice myocardium was observed;the level of cardiomyocyte apoptosis in mice was detected;mRNA expression levels of B-cell lymphoma 2(Bcl-2),Bcl-2 associated X protein(Bax),and Cleaved-caspase-3 in myocardial tissue of mice were detected;the phosphorylation levels of sarcoplasmic reticulum calcium regulatory related proteins[ryanodine receptor 2(RyR2)and phospholamban(PLB)]in myocardial tissue of mice were detected.RESULTS Compared with the blank group,the body weight,total distance of open field action,swimming exhaustion time,LVEF,LVEDD,Bcl-2 mRNA expression level in myocardial tissue and PLB protein phosphorylation level in the model group were significantly reduced/shortened(P＜0.05);TCM syndrome score,the percentage of immobility time,heart rate,LVESD,left ventricular mass index,whole heart mass index,cardiomyocyte apoptosis rate,the contents of CK,LDH and AST in serum,mRNA expression levels of Cleaved-caspase-3 and Bax and the phosphorylation level of RyR2 protein in myocardial tissue were significantly increased(P＜0.05);there were inflammatory cell infiltration,disordered cell arrangement and obvious myocardial interstitial fibrosis in myocardial tissue.After the intervention of Shengmai powder,most of the above quantitative indexes in mice were significantly reversed(P＜0.05),the inflammatory cell infiltration in myocardial tissue was reduced,and the degree of fibrosis was significantly reduced.CONCLUSIONS Shengmai powder can improve cardiac function,reduce the level of cardiomyocyte apoptosis and myocardial fibrosis in HF mice with qi-yin deficiency.Its mechanism may be related to the regulation of the expression of sarcoplasmic reticulum calcium regulation related proteins.关键词
生脉散/气阴两虚/心力衰竭/雷尼丁受体2/受磷蛋白/肌质网Key words
Shengmai powder/qi-yin deficiency/heart failure/ryanodine receptor 2/phospholamban/sarcoplasmic reticulum分类
医药卫生引用本文复制引用
康兰芳,李建,赵雅婷,陈迎春,陈贵银,聂晓博,刘姣,程杰..生脉散对气阴两虚型心力衰竭小鼠的改善作用及机制研究[J].中国药房,2025,36(17):2127-2133,7.基金项目
河北省中医药管理局科研计划项目(No.2022352) （No.2022352）
