中国药房2025，Vol.36Issue(17)：2134-2140,7.DOI:10.6039/j.issn.1001-0408.2025.17.09
枸杞多糖抑制口腔癌细胞增殖、迁移及免疫逃逸的机制研究
Study on the mechanism of Lycium barbarum polysaccharides inhibiting the proliferation,migration and immune escape of oral cancer cells
摘要
Abstract
OBJECTIVE To explore the effects of Lycium barbarum polysaccharides on the proliferation,migration,and immune escape of oral cancer cells by regulating the T cell immunoglobulin and mucin domain-containing protein 3(TIM3)/galectin-9(Gal-9)signaling pathway.METHODS Human oral cancer cells KB and CAL27 were assigned to control group,L.barbarum polysaccharides low-concentration group(200 μg/mL),L.barbarum polysaccharides high-concentration group(400 μg/mL),pcDNA-NC group(transfection of pcDNA-NC plasmid),pcDNA-TIM3 group(transfection of pcDNA-TIM3 plasmid),high concentration of L.barbarum polysaccharides+pcDNA-NC group(400 μg/mL L.barbarum polysaccharides+transfection of pcDNA-NC plasmid),and high concentration of L.barbarum polysaccharides+pcDNA-TIM3 group(400 μg/mL L.barbarum polysaccharides+transfection of pcDNA-TIM3 plasmid).The proliferation,migration and invasion abilities of the cells,T cell killing rate as well as the levels of interferon-γ(IFN-γ)and interleukin-2(IL-2)in the cell supernatant were measured.mRNA expressions of TIM3 and Gal-9 and protein expressions of indoleamine 2,3-dioxygenase 1(IDO1),programmed death-ligand 1(PD-L1),TIM3 and Gal-9 in the cells were also determined.RESULTS Compared with control group,the clone formation rate,scratch healing rate,the number of invasive cells,protein expressions of IDO1 and PD-L1,mRNA and protein expressions of TIM3 and Gal-9 in both cell types of L.barbarum polysaccharide low-and high-concentration groups were decreased significantly(P＜0.05),while the proliferation inhibition rate,T cell killing rate,and the levels of IFN-γ and IL-2 were significantly increased(P＜0.05).Compared with control group and pcDNA-NC group,the clone formation rate,scratch healing rate,the number of invasive cells,and protein expressions of IDO1 and PD-L1,mRNA and protein expressions of TIM3 and Gal-9 in both cell types of the pcDNA-TIM3 group were all significantly increased(P＜0.05),while the proliferation inhibition rate,T cell killing rate,IFN-γ and IL-2 levels were significantly decreased(P＜0.05).Compared with L.barbarum polysaccharides high-concentration group and high concentration of L.barbarum polysaccharides+pcDNA-NC group,the clone formation rate,scratch healing rate,the number of invasive cells,and protein expressions of IDO1 and PD-L1,mRNA and protein expressions of TIM3 and Gal-9 in both cell types of high concentration of L.barbarum polysaccharides+pcDNA-TIM3 group were significantly increased(P＜0.05),while the proliferation inhibition rate,T cell killing rate,and the levels of IFN-γ and IL-2 were significantly decreased(P＜0.05).CONCLUSIONS L.barbarum polysaccharides may inhibit the proliferation,migration,and immune escape of oral cancer cells by suppressing TIM3/Gal-9 signaling pathway.关键词
枸杞多糖/口腔癌/T细胞免疫球蛋白黏蛋白3/半乳糖凝集素9/增殖/迁移/免疫逃逸Key words
Lycium barbarum polysaccharides/TIM3/galectin-9/oral cancer/proliferation/migration/immune escape分类
医药卫生引用本文复制引用
李锦宇,徐晓雨,王钰卓..枸杞多糖抑制口腔癌细胞增殖、迁移及免疫逃逸的机制研究[J].中国药房,2025,36(17):2134-2140,7.基金项目
安徽省自然科学基金项目(No.2408085QB059) （No.2408085QB059）
