中国肿瘤生物治疗杂志2025，Vol.32Issue(8)：847-853,7.DOI:10.3872/j.issn.1007-385x.2025.08.008
肌营养不良蛋白-α在胃癌中的表达、功能及其调控机制
Expression,function and regulatory mechanisms of dystrobrevin-α in gastric cancer
摘要
Abstract
Objective:To investigate the expression characteristics,biological functions,and regulatory mechanisms of dystrobrevin-ɑ(DTNA)in gastric cancer tissues.Methods:Bioinformatics analysis based on public databases was used to predict DTNA expression in gastric cancer and its correlation with prognosis.Bioinformatics analysis based on public databases was used to predict DTNA expression in gastric cancer and its correlation with prognosis.qPCR and immunohistochemistry(IHC)were employed to detect DTNA expression in gastric cancer tissues and cells.The chi-square test analyzed the correlations between DTNA and clinicopathological features of gastric cancer,while Kaplan-Meier survival analysis assessed the relationship between DTNA expression and prognosis.CCK-8 and Transwell assays detected the effects of DTNA on the proliferation,migration,and invasion of gastric cancer MGC-803 cells.RNA interference,qPCR,and WB assay were used to examine the effects of far upstream element-binding protein 1(FUBP1)on DTNA expression in MGC-803 cells.Results:Bioinformatics analysis revealed upregulated DTNA expression in gastric cancer tissues versus adjacent tissues,correlating with poor prognosis.DTNA was upregulated in gastric cancer tissues(P<0.0001)and cells(P<0.05),showing positive correlations with T stage(P<0.001),TNM stage(P=0.001),and poor prognosis(Log-Rank P=0.0039).DTNA knockdown significantly inhibited the proliferation,migration,and invasion abilities of MGC-803 cells(all P<0.01).FUBP1 downregulation reduced DTNA expression(P<0.01).Conclusion:DTNA is upregulated in gastric cancer tissues and cells and is correlated with disease progression and poor prognosis.DTNA knockdown inhibits the proliferation,migration,and invasion of gastric cancer cells,and its expression is regulated by FUBP1.关键词
胃癌/增殖/迁移/侵袭/ɑ-肌营养不良蛋白相关蛋白基因/MGC-803细胞Key words
gastric cancer/proliferation/migration/invasion/dystrobrevin-ɑ(DTNA)/MGC-803 cell分类
医药卫生引用本文复制引用
孙艺萌,孙艺菲,卢慧雯,朱子锐,姚君桥,黄宝俊..肌营养不良蛋白-α在胃癌中的表达、功能及其调控机制[J].中国肿瘤生物治疗杂志,2025,32(8):847-853,7.基金项目
沈阳市科技计划项目(No.21-173-9-78) （No.21-173-9-78）
