中国肿瘤生物治疗杂志2025，Vol.32Issue(8)：854-861,8.DOI:10.3872/j.issn.1007-385x.2025.08.009
多能蛋白聚糖通过基质金属蛋白酶9促进肺腺癌细胞的恶性生物学行为
Versican promotes the malignant biological behaviors of lung adenocarcinoma cells via matrix metalloproteinase-9
摘要
Abstract
Objective:To investigate the roles and regulatory mechanisms of versican(VCAN)and matrix metalloproteinase-9(MMP9)in the invasion and metastasis of lung adenocarcinoma(LUAD).Methods:Thirty LUAD specimens and their matched adjacent non-tumor tissues were collected.Immunohistochemistry(IHC)was performed to detect VCAN and MMP9 expressions.Human LUAD cell lines NCI-H1975 and A549 were employed as models.siRNA interference and plasmid overexpression,combined with rescue assays,were applied.Cell proliferation,migration,and invasion abilities were evaluated by CCK-8,scratch-healing assay,and Transwell assay,respectively.Gene and protein expression were detected by RT-qPCR and WB assay.Results:VCAN and MMP9 were significantly up-regulated in LUAD tissues compared with those in adjacent non-tumor tissues(both P<0.001),and their H-score increased progressively with advancing tumor stage(both P<0.01).In vitro experiments revealed that si-VCAN markedly reduced VCAN and MMP9 mRNA and protein levels and suppressed cell viability,migration,and invasion(all P<0.01),whereas MMP9 overexpression significantly promoted these malignant phenotypes(all P<0.001);these effects were reversed by si-VCAN(all P<0.01).MMP9 knockdown did not significantly affect VCAN protein levels(P>0.05).Conclusion:VCAN enhances the proliferation,migration,and invasion of LUAD cells by up-regulating MMP9.The VCAN/MMP9 axis may represent a potential therapeutic target for LUAD.关键词
多能蛋白聚糖/迁移/侵袭/肺腺癌/基质金属蛋白酶9Key words
versican(VCAN)/migration/invasion/lung adenocarcinoma(LUAD)/matrix metalloproteinase-9(MMP9)分类
医药卫生引用本文复制引用
黄炜祺,黄瑜,刘小婷,代丹,何翔,董健..多能蛋白聚糖通过基质金属蛋白酶9促进肺腺癌细胞的恶性生物学行为[J].中国肿瘤生物治疗杂志,2025,32(8):854-861,8.基金项目
湖北省卫生健康委科研项目(No.WJ2023M148) （No.WJ2023M148）
武汉市卫生科研基金(No.WZ22Q01) （No.WZ22Q01）
华中科技大学协和江北医院自由创新预研基金(No.XHJBKXYJJJ-QNXM-06) （No.XHJBKXYJJJ-QNXM-06）
