北京林业大学学报2025，Vol.47Issue(9)：67-79,13.DOI:10.12171/j.1000-1522.20250088
林下植被叶际微生物群落对生活型与光照的响应
Responses of phyllosphere microbial communities in understory vegetation to plant life form and light intensity
摘要
Abstract
[Objective]Phyllosphere microbial communities have important impacts on forest ecosystem stability and plant health.This paper aims to reveal the effects of light intensity and plant life forms on phyllosphere microbial communities of understory vegetation,providing theoretical support for urban forest ecological management and ecosystem stability.[Method]Typical shrubs and herbs under Pinus tabuliformis plantations in Beijing Xishan National Forest Park were selected as research subjects.Based on photosynthetically active radiation(PAR),three light intensity gradients were established:low(<500 μmol/(m2·s)),medium(500-1 000 μmol/(m2·s)),and high(>1 000 μmol/(m2·s)).Using high-throughput sequencing,diversity,community structure,and amplicon sequence variant(ASV)-specific response characteristics of phyllosphere bacterial and fungal communities were analyzed.[Result](1)Light intensity had no significant effects on α-diversity of bacterial and fungal communities,whereas plant life forms significantly affected microbial diversity and structure,especially fungi.Chao1,Observed_species,and Good's_coverage indices of fungal communities in herbs were significantly higher than those in shrubs(p<0.05).PERMANOVA results showed plant life forms explained 3.83%and 3.70%of variations in bacterial and fungal communities,respectively(p<0.001).(2)Community composition analysis indicated bacterial communities were dominated by Proteobacteria,and their dominant genera remained stable across conditions.However,fungal communities varied significantly at the genus level with light intensity and plant type,with Phoma dominating under low-light conditions and Alternaria under medium and high light.PCoA and PERMANOVA analyses further verified significant impact of plant life forms on microbial community structure(p<0.001),while the effect of light intensity was not significant.(3)ASV analysis indicated high specificity among life forms and plant species;fungal communities shared 18.6%of ASVs between shrubs and herbs,while bacterial communities shared only 11.47%,indicating even higher specificity.At the species level,ASVs were mostly species-specific,showing strong host specificity.[Conclusion]Plant life form is a key driver of phyllosphere microbial community structure in understory vegetation,with a more pronounced effect than light intensity,especially on fungal assemblages.While bacterial communities remain relatively stable,fungal composition varies across light conditions and host types.These findings highlight the ecological role of life form in shaping microbial assemblages,offering a foundational reference for future research and the ecological design of urban forest systems.关键词
叶际微生物/林下植被/生活型/光照强度/城市森林Key words
interleaf microorganisms/understory vegetation/life form/light intensity/urban forest分类
农业科技引用本文复制引用
张子璇,孟晓倩,张新娜,徐程扬,陈涛,王文学,宁秋玲..林下植被叶际微生物群落对生活型与光照的响应[J].北京林业大学学报,2025,47(9):67-79,13.基金项目
国家自然科学基金项目(32271832),北京林业大学"5·5工程"科研创新团队项目(BLRC2023B06). （32271832）
