动物医学进展2025，Vol.46Issue(10)：35-42,8.
白头翁散对腹泻仔猪脑肠互动CRF和5-HT相关基因的影响
Effects of Pulsatilla Powder on CRF and 5-HT Related Genes of Brain-gut Interaction in Piglets with Diarrhea
摘要
Abstract
To investigate the regulatory effects of Pulsatilla Powder(pulsatilla powder,PP)on brain-gut axis of piglets with diarrhea,TRIzol reagent was used to extract total RNA from hypothalamus,hippocam-pus,ileum and colon tissues of piglets in each group and its contents were determined.The mRNA relative expression levels of CRF and 5-HT related genes in hypothalamus,hippocampus,ileum and colon tissues of piglets with diarrhea were analyzed by RT-qPCR.The results showed as follows:In the CRF-related genes,the mRNA expression level of CRFR-1 in ileum of Model group was increased,while the mRNA expression level of CRFR-2 in hippocampus and colon was decreased.After treatment,CRFR-1 mRNA expression lev-els in hypothalamus,ileum and colon of piglets in PP group were decreased,while CRFR-2 mRNA expres-sion levels in hypothalamus,hippocampus and ileum were increased.In the 5-HT related genes,the expres-sion level of 5-HT3R mRNA in the hippocampus of Model group was increased,the expression level of 5-HT4R mRNA was decreased in the ileum,the expression level of TPH1 mRNA was increased in the hy-pothalamus,but the expression level of the hippocampus was decreased.After treatment,the expression of 5-HT3R mRNA in the hippocampus and colon of piglets in PP group was decreased,the expression of 5-HT4R mRNA was increased in the ileum,the expression of TPH1 mRNA was increased in the ileum,and the expression of hypothalamus was decreased.The results indicated that Pulsatilla Powder could regulate the mRNA expression of CRF and 5-HT related genes in the brain and gut tissues of diarrheal piglets and affect the brain-gut interaction of diarrheal piglets,so as to improve the diarrhea status of piglets.关键词
白头翁散/腹泻仔猪/促肾上腺皮质激素释放因子/5-羟色胺/脑-肠轴Key words
Pulsatilla Powder/Piglets with diarrhea/CRF/5-HT/Brain-gut axis分类
农业科技引用本文复制引用
于滔,张昕杰,张丽芳,王晨颖,赖慧明,马琪..白头翁散对腹泻仔猪脑肠互动CRF和5-HT相关基因的影响[J].动物医学进展,2025,46(10):35-42,8.基金项目
国家自然科学基金青年科学基金项目(32202849) （32202849）
