动物医学进展2025，Vol.46Issue(10)：56-62,7.
70株猪链球菌的分离鉴定和毒力基因检测及耐药性分析
Isolation,Identification,Detection of Virulence Genes and Analysis of Drug Resistance of 70 Streptococcus suis Strains
摘要
Abstract
In order to know the distribution of Streptococcus suis from pigs in Baise,Guangxi,as well as the virulence genes carried by the strains and their resistance to commonly used antibiotics,the matrix assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS)was utilized to idenbtify the Streptococcus suis isolated from 366 healthy pigs' tonsil samples,which were acquired from 12 counties(cities,districts)in Baise,Guangxi.The virulence genes carried by the strains were detected by polymerase chain reaction(PCR),and the commonly used antimicrobial resistance analysis of the strains was carried out using micro broth dilution method.The result showed that,a sum of 70 isolated strains were identified as Streptococcus suis,with a sample carrying rate of 19.1％.The positive samples were mainly distributed along the Youjiang River axis and positively correlated with the transportation and circulation volume of pigs in the jurisdiction.The dominant virulence genotypes of the 70 Streptococcus suis strains were mainly about fbps,gapdh,orf2,and four strains carrying fbps,gapdh,orf2,mrp,sly,cps2J were detected,there was a slight discrepancy in the distribution of virulence genotypes among the strains from different i-solated counties(cities,districts).70 Streptococcus suis strains had the highest resistance rate to erythro-mycin and clindamycin,both of them were 94.3％,followed by tetracycline(92.3％),and the lowest resist-ance to ceftiaxone(2.8％).The overall resistance of isolated strains was serious,of which 62 strains showed multi-drug resistance,accounting for 88.6％,there were 34 strains being triple resistant,12 strains being fourfold resistant,11 strains being fivefold resistant,and five strains being sixfold resistant among them.The prevalent drug resistance pattern of the strains was triple resistance to clindamycin,erythromy-cin,and tetracycline.This study would be a reference to select the scientific clinical medication for preven-tion and treatment of swine streptococosis and promote the healthy development of pig industry.关键词
猪链球菌/基质辅助激光解吸电离飞行时间质谱/毒力基因/聚合酶链式反应/耐药Key words
Streptococcus suis/MALDI-TOF MS/Virulence gene/PCR/Drug resistance引用本文复制引用
韦文飞,袁朝原,李雪珍,农爱红,易雪丽,言天久,黄婵娟,黄伟坚..70株猪链球菌的分离鉴定和毒力基因检测及耐药性分析[J].动物医学进展,2025,46(10):56-62,7.基金项目
百色市科学技术局2022年百色市科学研究与技术开发计划课题项目(百科2022932) （百科2022932）
广西壮族自治区教育厅2021年度广西高校中青年教师科研基础能力提升项目(2021KY0545) （2021KY0545）
