南方医科大学学报2025，Vol.45Issue(9)：1840-1849,10.DOI:10.12122/j.issn.1673-4254.2025.09.04
清心牛黄丸通过改善脂质代谢紊乱缓解小鼠非酒精性脂肪性肝病
Ching Shum Pills alleviates non-alcoholic fatty liver disease in mice by ameliorating lipid metabolism disorders
摘要
Abstract
Objective To investigate the effect of Ching Shum Pills(CSP)for alleviating non-alcoholic fatty liver disease(NAFLD)and the underlying mechanism.Methods In a mouse model of NAFLD,the therapeutic effect of CSP was evaluated by measuring serum glucose,lipid profiles(TC,TG,LDL-C,HDL-C),and hepatic function markers.Network pharmacology was employed to identify active compounds in CSP and their targets using TCMSP,HERB,SwissTargetPrediction,GeneCards,OMIM,and DisGeNET.Protein-protein interaction(PPI)networks,Gene Ontology(GO),and KEGG pathway analyses were conducted.Molecular docking(AutoDock Vina)was used to assess the compound-target binding affinities.Quantitative real-time PCR(qRT-PCR)was used to validate the mRNA expressions of the core genes in the liver tissue of the mouse models.Results In the mouse model of NAFLD,treatment with CSP significantly reduced body weight gain and serum TG levels of the mice,and high-dose CSP treatment resulted in obvious reduction of ALT levels and hepatic fat accumulation.Network pharmacology analysis identified quercetin and 2-monolinolenin as the key bioactives in CSP,which target TNF,AKT1,IL6,TP53,and ALB.Docking simulations suggested strong binding between the two core compounds and their target proteins.The results of qRT-PCR showed that high-fat diet induced significant downregulation of Tp53,Cpt1,and Ppara expressions in mice,which was effectively reversed by CSP treatment.Conclusion CSP can improve lipid metabolism disorders in NAFLD mice through a regulatory mechanism involving multiple targets and pathways to reduce liver fat accumulation and protect liver function.The key components in CSP such as quercetin and linolenic acid monoacylglycerol may participate in the regulation of such metabolic processes as fatty acid oxidation by targeting TP53.关键词
非酒精性脂肪性肝病/清心牛黄丸/槲皮素/脂质代谢/肝功能Key words
non-alcoholic fatty liver disease/Ching Shum Pills/quercetin/lipid metabolism/liver function引用本文复制引用
骆碧云,易欣,蔡怡静,张世卿,王鹏,李彤,翁建霖,周平正..清心牛黄丸通过改善脂质代谢紊乱缓解小鼠非酒精性脂肪性肝病[J].南方医科大学学报,2025,45(9):1840-1849,10.基金项目
国家自然科学基金与香港研究资助局合作研究项目(8231101099) （8231101099）
香港研究资助局研究配对补助金计划(RMGS2021-10-13,RMGS2023-5-012) （RMGS2021-10-13,RMGS2023-5-012）
