南方医科大学学报2025，Vol.45Issue(9)：1859-1866,8.DOI:10.12122/j.issn.1673-4254.2025.09.06
中国人群外周血MLPH基因高甲基化与冠心病相关
Association between MLPH gene hypermethylation in peripheral blood and coronary heart disease
摘要
Abstract
Objective To investigate the association between methylation levels of tumor suppressing subtransferable candidate 1(TSSC1)and melanophilin(MLPH)genes in peripheral blood and coronary heart disease(CHD)in Chinese population.Methods This case-control study was conducted in 86 CHD patients and 95 healthy individuals,whose methylation levels of TSSC1 and MLPH genes in peripheral blood were determined using mass spectrometry.Mann-Whitney U test was used to compare the methylation levels in different subgroups.The correlation of TSSC1 and MLPH gene methylation levels with age and gender were evaluated using Spearman correlation coefficient and contingency coefficient,respectively.Results Compared with the healthy individuals,the CHD patients showed a significant correlation between MLPH hypermethylation and myocardial infarction(MI)(MLPH_CpG_2.7:P=0.045;MLPH_CpG_3/cg06639874:P=0.049;MLPH_CpG_5:P=0.019),and this correlation was even stronger in individuals below 65 years of age(MLPH_CpG_2.7:P=0.014;MLPH_CpG_4:P=0.001)and in male subjects(MLPH_CpG_2.7:P=0.004;MLPH_CpG_3/cg06639874:P=0.044).The methylation level of TSSC1 gene in peripheral blood was not found to correlate with CHD or its subtypes.Conclusion Our findings suggest a correlation of MLPH hypermethylation in peripheral blood with CHD and MI in Chinese population,especially in individuals below 65 years and in male individuals.关键词
冠心病/TSSC1/MLPH/DNA甲基化/外周血Key words
coronary heart disease/TSSC1/MLPH/DNA methylation/peripheral blood引用本文复制引用
金家列,王菲,朱丽雅,赵晓静,王金鑫,朱超,杨蓉西..中国人群外周血MLPH基因高甲基化与冠心病相关[J].南方医科大学学报,2025,45(9):1859-1866,8.基金项目
国家自然科学基金(82000311) （82000311）
江苏特聘教授科研基金项目(KY103R201938) Supported by National Natural Science Foundation of China(82000311). （KY103R201938）
