南方医科大学学报2025，Vol.45Issue(9)：1997-2005,9.DOI:10.12122/j.issn.1673-4254.2025.09.19
高度近视白内障患者晶状体前囊膜中circRNAs的差异性表达
Differential expression of circRNAs in anterior lens capsules of high myopic patients with cataract
摘要
Abstract
Objective To analyze the differential expression and biological functions of circRNAs in the anterior lens capsules of high myopic patients with cataract and their pathogenic roles in the development of this condition.Methods Anterior lens capsule specimens were collected intraoperatively from 36 patients with age-related cataract(ARC)and 36 high myopic patients with cataract.Among these,18 specimens from each group were selected for whole transcriptome sequencing and biological analysis,and the remaining 36 specimens were used for validation of circPDGFRA,circFOXJ3,hsa_circ_0004767,hsa_circ_0007528,ciCRIM1,circMAN1A2,circSLC5A3,and circPTK2 expressions using RT-qPCR.hsa_circ_0007528 was selected for cell experiments to examine its effects on proliferation,migration,and apoptosis of lens epithelial cells(LECs).Results A total of 16 192 circRNAs were detected in the specimens from both groups,among which 62 circRNAs were differentially expressed(29 upregulated and 33 downregulated).GO and KEGG analyses revealed that the differentially expressed circRNAs were primarily localized in the cytoplasm,nucleoplasm,and endoplasmic reticulum,and were involved in signaling pathways associated with Gap junction and the PI3K-Akt,NF-κB,Jak-STAT,HIF-1,and MAPK signaling pathways.The ceRNA network predicted multiple target genes.RT-qPCR validation results were consistent with the sequencing data.In the LECs,upregulation of hsa_circ_0007528 significantly inhibited cell proliferation and migration and obviously promoted cell apoptosis.Conclusion The expression profile of circRNAs in the anterior lens capsule of high myopic patients with cataract differs from that of ARC patients.Upregulation of hsa_circ_0007528 inhibits LEC proliferation and migration and promotes cell apoptosis.关键词
高度近视白内障/circRNA谱/全转录组测序/hsa_circ_0007528/晶状体上皮细胞Key words
high myopia with cataract/circRNA spectrum/whole transcriptome sequencing/hsa_circ_0007528/lens epithelial cells引用本文复制引用
韩圆圆,孙凤,刘艳,徐梦月,许澈,李娜,李娟,王剑锋..高度近视白内障患者晶状体前囊膜中circRNAs的差异性表达[J].南方医科大学学报,2025,45(9):1997-2005,9.基金项目
安徽省临床医学研究转化专项(202427b10020008) （202427b10020008）
安徽高校自然科学研究重点项目(KJ2021A0718) （KJ2021A0718）
