动物医学进展2025，Vol.46Issue(10)：20-25,6.
双峰驼血浆外泌体对肝癌增殖和侵袭的影响
Effects of Exosomes from Bactrian Camel Plasma on the Proliferation and Invasion of Liver Cancer
摘要
Abstract
To explore the effects of exosomes in Bactrian camel plasma on the proliferation and invasion of hepatocellular carcinoma(HCC)cells,in this experiment,exosomes in Bactrian camel plasma were extrac-ted by the ultracentrifugation method.Transmission electron microscopy and nanoparticle tracking analysis technology were used to observe and identify their morphological characteristics.Meanwhile,an exosome cell tracing experiment was carried out to evaluate the uptake of exosomes by HCC cells(HEP3B-luc).Subsequently,the mimics of miR-144-5p with significantly differential expression in the exosomes and the miR-144-5p negative control(mimics NC)were transfected into HEP3B-luc cells.The CCK-8 assay and cell invasion assay were employed to detect the effects of miR-144-5p on the proliferation and invasion of HEP3B-luc cells.The results showed that the extracted exosomes were cup-shaped or round,with a size distribution ranging from 50 nm to 200 nm,and HEP3B-luc cells could effectively take up the exosomes.The results of the CCK-8 assay indicated that,compared with the control group,the proliferation ability of HEP3B-luc cells in the mimics group was significantly decreased(P＜0.05),while there was no significant difference in the mimics NC group(P＞0.05).The results of the cell invasion assay showed that the inva-sion ability of HEP3B-luc cells in the mimics group was significantly lower than that in the control group(P＜0.01),while there was no significant difference in the mimics NC group.The above results suggest that miR-144-5p can significantly inhibit the proliferation and invasion abilities of HEP3B-luc cells.关键词
双峰驼/血浆外泌体/miR-144-5p/肝癌/细胞增殖/细胞侵袭Key words
Bactrian camel/Plasma exosomes/miR-144-5p/Hepatocellular carcinoma/Cell proliferation/Cell invasion引用本文复制引用
李泽旭,阿拉腾苏布德,斯日古楞,牛雅如,贺星川,金君健,李子怡,么宏强..双峰驼血浆外泌体对肝癌增殖和侵袭的影响[J].动物医学进展,2025,46(10):20-25,6.基金项目
内蒙古自治区自然科学基金项目(2024MS03071,2023MS03021) （2024MS03071,2023MS03021）
国家自然科学基金项目(32160821) （32160821）
