干旱区研究2025，Vol.42Issue(9)：1640-1649,10.DOI:10.13866/j.azr.2025.09.08
梭梭种子内生微生物群落组成及功能
Community composition and functionalities of endophytic microorganisms in Haloxylon ammodendron seeds
摘要
Abstract
This study aimed to reveal the ecological adaptive mechanisms and agricultural potential of endophytic microorganisms in desert plant seeds,using Haloxylon ammodendron seeds as the research subject.We combined high-throughput sequencing and conventional culture techniques to analyze the structural characteristics of endo-phytic microbial communities in H.ammodendron seeds and elucidate their potential roles in plant growth promo-tion and stress resistance.The results revealed that the endophytic bacterial community in H.ammodendron seeds comprised 668 species spanning 31 phyla,dominated by Firmicutes and Bacteroidetes,whereas the endophytic fungal community included 583 species spanning 13 phyla,dominated by Ascomycota and Basidiomycota.Func-tional annotation revealed distinct metabolic roles:the endophytic bacteria were enriched in chemoheterotrophy and fermentation,whereas the fungal communities exhibited saprophytic and pathogenic traits.Using convention-al culture methods,we isolated 13 culturable endophytic bacterial strains,including two multifunctional plant growth-promoting strains(Priestia aryabhattai HB-4 and Priestia megaterium HB-9)and three salt-tolerant strains(Bacillus zhangzhouensis HB-6,Bacillus safensis HB-10,and Bacillus pumilus HB-11).Pot experiments demonstrated that HB-4 and HB-9 significantly enhanced wheat growth,while HB-6,HB-10,and HB-11 alle-viated saline-alkaline stress in wheat.This study elucidates the ecological roles of endophytic microbes in H.am-modendron seeds,providing novel insights into the exploitation of microbial resources in desert ecosystems and the development of plant growth-promoting microbial agents.关键词
梭梭种子/内生微生物/群落结构/多样性/促生菌Key words
Haloxylon ammodendron seeds/endophytic microorganisms/community structure/diversity/growth-promoting bacterial引用本文复制引用
朱朝华,翟祎笑,李欣荣,缪潆祥,马彤,李善家..梭梭种子内生微生物群落组成及功能[J].干旱区研究,2025,42(9):1640-1649,10.基金项目
甘肃省重点研发计划项目(25YFFA043) （25YFFA043）
