赣南医科大学学报2025，Vol.45Issue(8)：733-738,6.DOI:10.3969/j.issn.1001-5779.2025.08.002
羊栖菜多酚抗氧化及细胞损伤保护作用研究
Study on antioxidant and cell damage protection effects of Sargassum fusiforme polyphenols
摘要
Abstract
Objective:Exploring the in vitro antioxidant activity of polyphenols from Sargassum fusiforme and their protective effects against H2O2-induced damage in human aortic endothelial cells(HAECs),thereby providing a theoretical basis for their application and development in functional food and pharmaceutical fields.Methods:Polyphenols from Sargassum fusiforme were extracted from Sargassum fusiforme with 60%ethanol,followed by defatted with petroleum ether and purification via silica gel column chromatography.The in vitro antioxidant capacity was assessed by measuring scavenging activities against 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals,superoxide anion(O2⁻)radicals,and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS+)radicals.An oxidative damage model was established in HAECs induced by H2O2 to evaluate the effects of polyphenols from Sargassum fusiforme on cell viability and analyze the dose-response relationship.Results:The polyphenols from Sargassum fusiforme showed significant scavenging ability to all three free radicals,and its half-maximal inhibitory concentration(IC50)was:DPPH radical 0.074 mg·mL-1,O₂⁻ radical 0.069 mg·mL-1,ABTS+radical 0.089 mg·mL-1.In cellular experiments,polyphenols from Sargassum fusiforme alleviated H2O2-induced oxidative damage in HAECs in a concentration-dependent manner and significantly enhanced cell viability(P＜0.05).Conclusion:The polyphenols from Sargassum fusiforme exhibit potent in vitro antioxidant activity and obvious cytoprotection.They act as natural antioxidants for functional foods or biomedical applications to prevent oxidative stress-related diseases.关键词
羊栖菜/多酚/抗氧化活性/细胞损伤Key words
Sargassum fusiforme/Polyphenols/Antioxidant activity/Cell damage分类
轻工纺织引用本文复制引用
陈琼,邱小明..羊栖菜多酚抗氧化及细胞损伤保护作用研究[J].赣南医科大学学报,2025,45(8):733-738,6.基金项目
漳州市食品产业技术研究院开放项目(ZSY2022105) （ZSY2022105）
漳州市自然科学基金资助项目(ZZ2020J43) （ZZ2020J43）
