iFSP1对SKOV3细胞铁死亡的影响及机制研究OA

Objective To explore the impact and underlying mechanisms of ferroptosis suppressor protein 1 inhibitor(iFSP1)on ferroptosis in SKOV3 cells,aiming to provide a novel theoretical foundation for clinical treatment strategies of ovarian cancer.Methods The effects of iFSP1 on cell morphology and growth status were observed using optical microscopy.The CCK-8 as-say was employed to evaluate the impact of iFSP1 alone or in combination with the ferroptosis inhibitor Lip-1 on cell viability.Intracel-lular levels of reactive oxygen species(ROS),were quantitatively analyzed using an ROS detection kit.Western blotting was per-formed to measure the expression levels of FSP1 protein in cells treated with iFSP1.Additionally,the CCK-8 assay was used to assess the effects of ferroptosis inducer RSL3 alone or in combination with iFSP1 on cell viability.Results iFSP1 significantly inhibited the proliferation of SKOV3 cells,with the inhibition rate increasing progressively with higher concentrations of iFSP1 and longer treatment durations(P<0.05).The ferroptosis inhibitor Lip-1 attenuated the inhibitory effect of iFSP1 on SKOV3 cells(P<0.05).iFSP1 markedly elevated ROS levels in the SKOV3 cells and downregulated the expression of FSP1 protein(P<0.05).Furthermore,iFSP1 enhanced the growth-inhibitory effect of the ferroptosis inducer RSL3 on SKOV3 cells(P<0.05).Conclusion iFSP1 induces fer-roptosis in SKOV3 cells by downregulating FSP1 protein expression,thereby inhibiting SKOV3 cells proliferation.Additionally,iFSP1 enhances the inhibitory effect of the ferroptosis inducer RSL3 on SKOV3 cells.