贵州农业科学2025，Vol.53Issue(9)：59-67,9.DOI:10.3969/j.issn.1001-3601.2025.09.008
盐胁迫下壳聚糖与枯草芽孢杆菌对番茄幼苗生长特性的影响
Effects of Chitosan and Bacillus subtilis Combination Treatment on Growth Characteristics of Tomato Seedlings Under Salt Stress
摘要
Abstract
[Objective]The effects of chitosan and Bacillus subtilis combination treatment on growth characteristics of tomato seedlings under salt stress were investigated to provide a reference for formulating cultivation measures of tomato under salt stress.[Method]Taking tomato seedings as test material,the agronomic traits,physiology and biochemistry,and soil sucrase and urease activity of tomato seedlings of 8 treatments including CK(no applying chitosan and B.subtilis),T1(4 mg/L B.subtilis),K1(100 mg/L chitosan),K2(200 mg/L chitosan),K3(300 mg/L chitosan),H1(100 mg/L chitosan+4 mg/L B.subtilis),H2(200 mg/L chitosan+4 mg/L B.subtilis)and H3(300 mg/L chitosan+4 mg/L B.subtilis)were determined under salt stress by using the foliage spray of chitosan(5 mL/seedling)and root-irrigating of B.subtilis(4 mL/seedling).[Result]Different chitosan and B.subtilis combination treatments had the significant effects on growth and relative enzyme activity of tomato seedlings.The height and stem diameter of H3 tomato seedlings were 31.50 cm and 6.43 cm on the 2nd day under salt stress,and 15.94%and 16.49%higher than CK respectively.The leaf chlorophyll relative content of H3 and T1 increased by 20.48%and 20.39%compared with CK.The agronomic traits of tomato seedlings under the treatment of B.subtilis and high-concentration chitosan(300 mg/L)were the best.And among the contentration treatment,the effect of low-concentration chitosan(100 mg/L)was lower than that of middle and high contentration treatment.The leaf SOD activity of H3 reached 75.39 U/g(the highest)on the 6th day under salt stress and was 58.98%higher than CK.The leaf MDA content of H3 was 17.90 nmol/g(the lowest)and 52.82%lower than CK.The leaf CAT activity of H1 tomato seedlings was the highest(585.79 U/g)and 50.92%higher than CK.The T1,K1,K2 and K3 treatments all could increase leaf SOD and CAT activity,and reduce leaf MDA content.The leaf SOD activity of H1,H2 and H3 tomato seedlings increased with increase of chitosan concentration as the leaf MDA content decreased.The leaf CAT activity of H2 tomato seedlings was lower than H1 and H3 with increase of chitosan concentration.As for soil enzyme activity,the sucrase activity of H3 was the highest(195.09 U/g)and 77.63%higher than CK.The urease activity of H2 was the highest(419.15 U/g)and 64.28%higher than CK.The T1,K1,K2 and K3 treatments all could increase soil sucrose and urease activity.The sucrose activity of H1,H2 and H3 increased with increase of chitosan concentration while the urease activity showed a rising first and then declining trend.[Conclusion]The chitosan and B.subtilis combination treatment has the promoting effects on growth,leaf enzyme activity and soil enzyme activity of tomato seedlings.The 300 mg/L chitosan+4 mg/L B.subtilis combination treatment has the significant effects on promoting morphological development and enhancing the antioxidant system of tomato seedlings,and improving soil enzyme activity under salt stress,indicating that it should be selected as the optimization formula for tomato seedling cultivation in saline-alkali soils.关键词
壳聚糖/枯草芽孢杆菌/番茄/盐胁迫/幼苗生长特性Key words
chitosan/Bacillus subtilis/tomato/salt stress/growth characteristics of seedling分类
农业科技引用本文复制引用
丁栋,王方静,史静..盐胁迫下壳聚糖与枯草芽孢杆菌对番茄幼苗生长特性的影响[J].贵州农业科学,2025,53(9):59-67,9.基金项目
2024年云南农业大学第十七届学生科技创新创业行动基金项目(2024Y0739) （2024Y0739）
