贵州农业科学2025，Vol.53Issue(9)：68-75,8.DOI:10.3969/j.issn.1001-3601.2025.09.009
务川黑牛TMEM-68基因多态性及其与生长性状的关联性研究
Correlation Study of Polymorphisms of TMEM-68 Gene with Growth Traits in Wuchuan Black Cattle
摘要
Abstract
[Objective]The correlation between polymorphisms of the transmembrane protein 68 gene(TMEM-68)and growth traits in Wuchuan black cattle were studied,which provided references for screening the domain group of Wuchuan black cattle and protecting the germplasm resource of cattle.[Method]Taking Wuchuan black cattle as study object,PCR and lluminaX-10 high-throughput sequencing were employed to obtain the complete sequence of the TMEM-68 gene in Wuchuan black cattle.Futhermore,the single nucleotide polymorphisms(SNPs)in the TMEM-68 gene associated with growth traits were analyzed,and the superior allelic variants were explored.[Result]8 SNP sites significantly associated with growth traits were detected,including 224(T/A),360(G/A),447(T/C),557(G/T),938(A/G),1135(A/G),1319(G/A),and 1567(T/A)loci of TMEM-68 gene in Wuchuan black cattle.The polymorphic loci of 8 SNP sites owned a high degree of homozygosity(0.65-0.71),showing low polymorphism(PIC＜0.25).At 224(T/A)site,the chest width and body weight of TT genotype individual were significantly higher than those of TA and AA.At the 360(G/A)site,the height of cross section,chest circumference and body height of AA genotype individual was significantly than GA.At the 447(T/C)site,the body height,height of cross section and chest width of CC genotype individual were significantly higher than TT.At the 557(G/T)site,the body diagonal length of GT and TT genotype individual were significantly higher than GG.At the 938(A/G)site,the chest width of GG and AA genotype individual were obviously higher than AG.At the 1135(A/G)site,the pin bone width of AA and GG genotype individual were significantly higher than AG.At the 1319(G/A)site,the height of cross section of GA and AA genotype individual were obviously higher than GG.At the 1567(T/A)site,the chest width of TT and TA were significantly than AA.Haplotype analysis demonstrated that there were 7 haplotypes in Wuchuan black cattle population,among which the number of haplotypes of H1 were significantly more than other haplotype combinations,indicating that it was the dominant haplotype.The analysis about haplotypes combination showed that H2H6 haplotype had great advantages in the height of cross section,body diagonal length,canon circumference and width of pin bone,and H1H7 haplotype had advantages in chest width,body height and body weight.[Conclusion]TMEM-68 gene was closely related to the growth traits of Wuchuan black cattle,could serve as a candidate molecular marker for the development and breeding of genetic resources of Wuchuan black cattle.关键词
务川黑牛/跨膜蛋白基因/单核苷酸多态性位点/生长性状/lluminaX-10高通量测序平台/分子标记/单倍型Key words
Wuchuan black cattle/transmembrane protein gene/SNPs/growth traits/lluminaX-10 high-through-put sequencing platform/molecular marker/haplotype分类
农业科技引用本文复制引用
谭尚琴,徐龙鑫,陈燕,武俊达,杨丽娟,王鑫,周文章,赵元峰,王华..务川黑牛TMEM-68基因多态性及其与生长性状的关联性研究[J].贵州农业科学,2025,53(9):68-75,8.基金项目
贵州省科技重大专项"贵州黄牛产业化发展关键技术研究与示范"(黔科合重大专项字[2020]3009) （黔科合重大专项字[2020]3009）
贵州省科技计划项目"贵州地方黄牛种质资源挖掘与创新利用"(黔科合支撑[2022]重点027) （黔科合支撑[2022]重点027）
贵州省农业科学院"揭榜挂帅"项目"肉牛性控冻精生产关键技术研究与应用"(黔农科JBGS[2024]02号) （黔农科JBGS[2024]02号）
