临床与病理杂志2025，Vol.45Issue(6)：664-672,9.DOI:10.11817/j.issn.2095-6959.2025.220049
CHST7和SHH在侵袭性无功能垂体神经内分泌肿瘤中的表达及其与临床病理特征的关系
Expression of CHST7 and SHH in invasive non-functioning pituitary neuroendocrine tumors and their association with clinicopathological features
摘要
Abstract
Objective:Identifying biomarkers to predict the invasiveness of non-functioning pituitary neuroendocrine tumors(PitNET)to guide early clinical management is a current research focus.Carbohydrate sulfotransferase(CHST)7 and Sonic hedgehog(SHH)are associated with the occurrence and progression of various tumors.This study aims to investigate the expression of CHST7 and SHH in invasive non-functioning PitNET and their relationship with clinicopathological features. Methods:A retrospective analysis was conducted on 52 patients with non-functioning PitNET treated at the Neurosurgery Department of Tangshan People's Hospital.Based on preoperative MRI and Knosp classification,patients were divided into an invasive group(n=28)and a non-invasive group(n=24).Expression levels of CHST7 and SHH in serum and tumor tissues were detected by enzyme-linked immunosorbent assay(ELISA)and immunohistochemistry.Their associations with clinicopathological features(Ki-67,p53,and lineage-specific transcription factor expression)were further analyzed. Results:Preoperatively,serum CHST7 and SHH protein levels in the invasive group were significantly higher than those in the non-invasive group(both P<0.05).Three weeks postoperatively,serum CHST7 and SHH levels decreased in both groups but remained significantly higher in the invasive group(both P<0.05).In invasive non-functioning PitNET,patients with Ki-67≥3％had higher CHST7 and SHH positivity than those with Ki-67<3％(both P<0.05).Strong p53-positive patients also showed higher CHST7 and SHH positivity compared to weak or negative p53 patients(both P<0.05).CHST7 positivity in patients with transcription factor 19(T-PIT)expression was significantly higher than that in patients without,while SHH positivity in patients with pituitary-specific POU-class homeodomain transcription factor-1(PIT1)expression was significantly higher than that in patients without(both P<0.05). Conclusion:High expression of CHST7 and SHH may be associated with the invasiveness of non-functioning PitNET.关键词
侵袭性/无功能垂体神经内分泌肿瘤/碳水化合物磺基转移酶7/音猬因子/Ki-67/p53/转录因子Key words
invasiveness/non-functioning pituitary neuroendocrine tumors/carbohydrate sulfotransferase 7/Sonic hedgehog/Ki-67/p53/transcription factors引用本文复制引用
张利,董伟,董晓柳,陈玉娟,张于,张欢,卓红..CHST7和SHH在侵袭性无功能垂体神经内分泌肿瘤中的表达及其与临床病理特征的关系[J].临床与病理杂志,2025,45(6):664-672,9.基金项目
河北省自然科学基金(H2022105014).This work was supported by the Natural Science Foundation of Hebei Province,China(H2022105014). （H2022105014）
