农业科技与技术（英文）2025，Vol.26Issue(2)：7-17,11.DOI:10.16175/j.cnki.1009-4229.2025.02.002
不同比例有机肥替代化肥对红壤稻田理化性状与细菌群落特征的影响
Effect of Different Proportions of Organic Fertilizer Replacing Chemical Fertilizer on the Physicochemical Properties and Bacterial Community Characteristics of Reddish Paddy Fields
摘要
Abstract
This study aimed to explore the effect of different proportions of organic fertilizer replacing chemical fertilizer on the bacterial community and metabolic function in paddy fields.The 16S rRNA absolute quantitative sequencing method was employed to study the response characteristics of soil bacterial community composition and species absolute abundance to environmental factors under three fertilization treatments[chemical fertilizer(NPK),organic fertilizer replacing 30%of chemical fertilizer(30M,estimated in terms of pure nitrogen,same below),and organic fertilizer replacing 60%of chemical fertilizer(60M)]for two consecutive years.Furthermore,the changes of bacterial metabolic functions of different fertilization treatments were predicted by PICRUSt2.The results showed that replacing chemical fertilizer with organic fertilizer at different proportions significantly increased the total nitrogen(TN),total potassium(TK),hydrolyzable nitrogen(HN),soil organic carbon(SOC),and significantly decreased the soil bulk density(SBD).Moreover,60M demonstrated better performance than 30M.Different fertilization treatments did not cause significant difference in soil bacterial richness index(Chao1)or diversity index(Shannon)but significantly affected bacterial community composition and species abundance.Particularly,60M significantly increased the abundance of 227 species,and it increased the total bacterial abundance by 25.30%and 56.58%compared with NPK and 30M,respectively.Redundancy analysis and Spearman correlation analysis revealed that SOC,TN,and AK were the key factors for shaping specific bacterial community structures under different fertilization treatments.The 60M treatment significantly increased the abundance of bacterial species involved in xenobiotic biodegradation and metabolism,amino acid metabolism,and lipid metabolism,thus improving the metabolic functions of soil microorganisms.关键词
有机肥/红壤稻田/细菌/高通量测序/代谢功能Key words
Organic fertilizer/Reddish paddy field/Bacteria/High-throughput sequencing/Metabolic functions引用本文复制引用
崔新卫,吕真真,高鹏,陈梓勋,鲁耀雄,薛涛,彭福元..不同比例有机肥替代化肥对红壤稻田理化性状与细菌群落特征的影响[J].农业科技与技术（英文）,2025,26(2):7-17,11.基金项目
Supported by Natural Science Foundation of Changsha City(kq2208107) （kq2208107）
Open Fund of Key Laboratory,Ministry of Agriculture and Rural Affairs(2025KLASAUMARA-1) （2025KLASAUMARA-1）
Open Fund of Key Laboratory of Jiangxi Province(2025JPKLALIQE-1) （2025JPKLALIQE-1）
Agricultural Science and Technology Innovation Fund Project of Hunan Province(2023CX110,2022CX83) 长沙市自然科学基金项目(kq2208 107) （2023CX110,2022CX83）
农业农村部重点实验室开放基金(2025 KLASAUMARA-1) （2025 KLASAUMARA-1）
江西省重点实验室开放基金(2025JPKLALIQE-1) （2025JPKLALIQE-1）
湖南省农业科技创新资金项目(2023CX110,2022CX83) （2023CX110,2022CX83）
