农业科技与技术（英文）2025，Vol.26Issue(2)：25-36,12.DOI:10.16175/j.cnki.1009-4229.2025.02.004
伴矿景天对低温胁迫响应的转录组分析
Transcriptomic Analysis of Sedum plumbizincicola in Response to Low-Temperature Stress
摘要
Abstract
To investigate the response mechanisms of Sedum plumbizincicola under low-temperature stress,phenotypic traits and physiological parameters including superoxide dismutase(SOD)activity,peroxidase(POD)activity,and malondialdehyde(MDA)content were examined following 6-hour treatments at 22(control),10,and-2 ℃.Transcriptome analysis was subsequently conducted.The results showed that low-temperature stress significantly enhanced antioxidant enzyme activities(SOD and POD),along with osmotic adjustment-related indicators such as MDA content,soluble sugar and protein content,and relative electrolytic conductivity.After quality control and optimization of the transcriptome data,a total of 78 803 unigenes were obtained,with an average length of 871.13 bp and an N50 of 1 510 bp.Compared with the control,8 391 and 10 730 differentially expressed genes(DEGs)were identified under 10 and-2 ℃ treatments,respectively.Gene Ontology(GO)enrichment analysis indicated that these DEGs were mainly involved in responses to abiotic stimuli including temperature,DNA-binding transcription factor activity,and amino acid metabolic processes.In S.plumbizincicola,the activation of flavonoid biosynthesis and MAPK signaling pathways likely played a critical role in low-temperature response.Under low-temperature stress,the plant enhanced its stress resistance by significantly elevating antioxidant enzyme activities and accumulating osmolytes.Concurrently,transcriptomic analysis indicated that metabolic pathways such as flavonoid biosynthesis,MAPK signaling,nitrogen metabolism,and plant hormone signal transduction were crucial for S.plumbizincicola's adaptation to low-temperature stress.关键词
伴矿景天/低温胁迫/转录组Key words
Sedum plumbizincicola/Low temperature stress/Transcriptome引用本文复制引用
毛洋,陈希,王博,陈锦..伴矿景天对低温胁迫响应的转录组分析[J].农业科技与技术（英文）,2025,26(2):25-36,12.基金项目
Supported by National Key Research and Development Program of China(2022YFD1700104-2) （2022YFD1700104-2）
Hunan Science and Technology Talent Lifting Project(2023TJ-N19) （2023TJ-N19）
Hunan Ecological Environment Scientific Research Projects(HBKT-202222) 国家重点研发计划(2022YFD1700104-2) （HBKT-202222）
湖南省科技人才托举工程(2023TJ-N19) （2023TJ-N19）
湖南省环保科研项目(HBKT-202222) （HBKT-202222）
