解剖学杂志2025，Vol.48Issue(4)：282-286,封3,6.DOI:10.3969/j.issn.1001-1633.2025.04.002
雄激素对急性海马脑片神经元树突棘和突触蛋白的快速非基因组作用
Rapid non-genomic effects of androgen on dendritic spine density and synaptic proteins in neurons of acute hippocampal slices
李莎 1冯宝峰 2陈欢 1崔慧先1
作者信息
- 1. 河北医科大学,人体解剖学教研室,石家庄 050017||河北医科大学,神经科学中心,石家庄 050017||河北医科大学,河北省神经退行性疾病机制研究重点实验室,石家庄 050017
- 2. 河北医科大学,人体解剖学教研室,石家庄 050017
- 折叠
摘要
Abstract
Objective:To prepare acute hippocampal brain slices and investigate the non-genomic effects of testosterone(T),dihydrotestosterone(DHT)and testosterone BSA(T-BSA)on dendritic spine density and synaptic proteins[synaptophysin(SYN)and postsynaptic density protein 95(PSD95)]in hippocampal brain slices.Methods:The brains of 3-month-old SD male rats were extracted after anesthesia,and the hippocampal tissue was dissected in artificial cerebrospinal fluid to prepare 300 μm coronal slices.DAPI and PI staining were applied at 0,15,30,60 min and 120 min to evaluate neuronal viability.Acute hippocampal brain slices were randomly divided into control(CON),T(10 nmol/L),DHT(10 nmol/L)and T-BSA(0.36 nmol/L)groups.The density of dendritic spines was observed by Golgi-Cox staining,and the synaptic proteins SYN and PSD95 were examined through immunofluorescence histochemical staining and Western blotting after 1 h administration.Results:DAPI and PI staining showed that there was no significant difference in the percentage of active cells at all 5 time points.Golgi-Cox staining showed that the density of dendritic spines in T group,DHT group and T-BSA group was significantly increased compared with CON group.There was no significant difference in dendrite spine density among T group,DHT group and T-BSA group.Immunofluorescence histochemical staining and Western blotting assay revealed that compared with CON group,the expression of SYN and PSD95 protein in T group,DHT group and T-BSA group were significantly increased.There were no significant differences in the expression of SYN and PSD95 protein among T group,DHT group and T-BSA group.Conclusion:Acute hippocampal slices maintain excellent and stable neuronal viability within 2 h.Androgens increase the density of dendritic spines and the expression of synaptic proteins SYN and PSD95 in acute hippocampal brain slices through non-genomic effects.关键词
雄激素/海马脑片/树突棘/突触蛋白/非基因组效应Key words
androgens/hippocampal slices/dendritic spines/synaptic protein/non-genomic effect分类
医药卫生引用本文复制引用
李莎,冯宝峰,陈欢,崔慧先..雄激素对急性海马脑片神经元树突棘和突触蛋白的快速非基因组作用[J].解剖学杂志,2025,48(4):282-286,封3,6.
