解剖学杂志2025，Vol.48Issue(4)：287-292,298,7.DOI:10.3969/j.issn.1001-1633.2025.04.003
栀子苷通过抑制脊髓细胞凋亡、炎症反应和TLR4/NF-κB通路减轻神经病理性疼痛
Geniposide alleviates neuropathic pain by inhibiting spinal cord cell apoptosis,inflammatory response,and TLR4/NF-κB pathway
摘要
Abstract
Objective:To investigate the effects of geniposide(Gen)on the spontaneous pain behavior,pain threshold,apoptosis,inflammatory response,and toll-like receptor 4(TLR4)/nuclear transcription factor kappa B(NF-κB)pathway of spinal cord in neuropathic pain(NPP)model mice.Methods:Mice were randomly divided into sham surgery group,CCI group,and Gen group.The NPP model was established by chronic constriction injury(CCI)of the sciatic nerve.The Gen group was intraperitoneally injected with Gen for 7 consecutive days after surgery.The autogenous pain behavior score,paw withdrawal thermal latency(PWTL)and paw withdrawal cold latency(PWCL)of mice were observed.Immunofluorescence staining was used to detect the expression of cleaved caspase-3 in spinal cord.RT-qPCR was applied to detect the expression of TNF-α,IL-1β,IL-6,and IL-10 mRNA in the spinal cord.ELISA was adopted to determine the protein levels of TNF-α,IL-1β,IL-6,and IL-10 in the spinal cord.Western blotting was utilized to detect the expression of TLR4,p-NF-κB p65,and NF-κB p65 proteins in the spinal cord.Results:Compared with the model group,the Gen group mice showed reduced autogenous pain behavior score,elevated PWTL and PWCL,and decreased pain sensitivity.The expression of cleaved caspase-3 protein in the spinal cord was reduced in the Gen group,with reduced mRNA and protein expression levels of TNF-α,IL-1β,and IL-6,while the expression of IL-10 was increased at both mRNA and protein levels in the Gen group.The expression of TLR4 and p-NF-κB p65 proteins was reduced,with no difference in the expression of NF-κB p65,the ratio of p-NF-κB p65 to NF-κB p65 decreased,and the activity of the TLR4/NF-κB pathway was decreased.Conclusion:Gen can alleviate spinal cord cell apoptosis,inflammatory response,and TLR4/NF-κB pathway in NPP mice,exerting analgesic effects.关键词
栀子苷/神经病理性疼痛/炎症反应/TLR4/NF-κB通路/凋亡Key words
geniposide/neuropathic pain/inflammatory response/toll-like receptor 4/nuclear transcription factor kappa B pathway/apoptosis分类
医药卫生引用本文复制引用
杨思琪,陈泽彬,张莉..栀子苷通过抑制脊髓细胞凋亡、炎症反应和TLR4/NF-κB通路减轻神经病理性疼痛[J].解剖学杂志,2025,48(4):287-292,298,7.基金项目
辽宁省自然科学基金(20180530093) （20180530093）
