解剖学杂志2025，Vol.48Issue(4)：293-298,6.DOI:10.3969/j.issn.1001-1633.2025.04.004
黄芩苷调节TLR4/MyD88/NF-κB信号通路改善多重耐药铜绿假单胞菌肺炎大鼠肺损伤
Baicalein alleviates lung injury in rats with multidrug-resistant pseudomonas aeruginosa pneumonia by regulating the TLR4/MyD88/NF-κB pathway
摘要
Abstract
Objective:To investigate whether baicalin can improve lung injury in multidrug-resistant Pseudomonas aeruginosa(MDR-PA)pneumonia rats through the TLR4/MyD88/NF-κB signaling pathway.Method:40 rats were randomly divided into four groups:blank group(blank group),MDR-PA pneumonia rat model group(MDR-PA group),MDR-PA pneumonia rat group treated with baicalein(BI group),and MDR-PA pneumonia rat group treated with baicalein and TLR4 agonist lipopolysaccharide(BI+LPS group),with 10 rats in each group.The lung index(LI)and lung wet/dry weight ratio of rats in each group,inflammatory cytokine levels(detected by ELISA),and histopathological changes in lung tissue(evaluated with haematoxylin-eosin staining)were measured.Western blotting was applied to determine the protein expression of TLR4,MyD88,NF-κB p65,and p-NF-κB p65 in lung tissue.Comparison of mRNA expression of TLR4,MyD88,NF-κB p65,and p-NF-κB p65 in uterine tissue was detected by RT-qPCR.Results:Compared with the blank group,the lung index,wet/dry weight ratio,serum levels of IL-6,IL-1β,TNF-α,and the protein expression of TLR4 and MyD88,the ratio of p-NF-κB p65/NF-κB p65,and mRNA expression of TLR4,MyD88,and NF-κB p65 were significantly increased in the MDR-PA group.Compared with the MDR-PA group,such parameters in the BI group were significantly reduced.By contrast,these parameters in the BI+LPS group were significantly higher than those in the BI group.Conclusion:Baicalin may alleviate lung injury in MDR-PA pneumonia rats by inhibiting the TLR4/MyD88/NF-κB signaling pathway to suppress inflammatory response.关键词
黄芩苷/TLR4/MyD88/NF-κB信号通路/炎症/多重耐药菌铜绿假单胞菌/肺炎Key words
baicalin/TLR4/MyD88/NF-κB signaling pathway/inflammation/multidrug resistant Pseudomonas aeruginosa/pneumonia分类
医药卫生引用本文复制引用
陈艾萍,蔡雯铮,周宇婕..黄芩苷调节TLR4/MyD88/NF-κB信号通路改善多重耐药铜绿假单胞菌肺炎大鼠肺损伤[J].解剖学杂志,2025,48(4):293-298,6.基金项目
上海市长宁区医学硕博士创新人才基地项目(RCJD2021B01) （RCJD2021B01）
