农业科技与技术（英文）2025，Vol.26Issue(2)：52-59,8.DOI:10.16175/j.cnki.1009-4229.2025.02.007
施用井冈霉素后烟草叶际微生态变化规律
Changes of Phyllosphere Microbial Communities of Tobacco after Application of Validamycin
摘要
Abstract
Validamycin is often used to control the diseases caused by Rhizoctonia.To investigate the changes of phyllosphere microbial communities after the application of validamycin,we employed high-throughput sequencing to study the structure and diversity of phyllosphere microbial communities of diseased and healthy tobacco leaves.The results showed that the phyllosphere microbial community structure and diversity altered significantly after the application of validamycin.The diseased leaves showed a decline in Shannon index and rises in ACE and Chao1 indexes.The healthy leaves showed no significant change in Shannon index and increases in ACE and Chao1 indexes.The dominant genera in diseased tobacco leaves were Pseudomonas,Enterobacter,Agrobacterium,and Stenotrophomonas,which showed higher relative abundance than those in healthy leaves.After the application of validamycin,diseased tobacco leaves showcased decreased relative abundance of Pseudomonas and Enterobacter and increased relative abundance of Agrobacterium and Methylobacterium,while healthy leaves showed reduced relative abundance of Methylobacterium.The KEGG pathway enrichment analysis showed that the pathways of phyllosphere bacteria of diseased and healthy tobacco leaves mainly had two categories of metabolism and genetic information processing,with differences in relative abundance.The results revealed the changes of phyllosphere microbial community structure and diversity after the application of validamycin and provided a reference for delving into the microecological mechanism of plant disease prevention and control by fungicides.关键词
烟草靶斑病/井冈霉素/叶际微生态/高通量测序Key words
Tobacco target spot/Validamycin/Phyllosphere microbial community/High-throughput sequencing引用本文复制引用
范才银,苏亮,姚强,刘勇,李建华,钟杰,李晓刚,黎萍,肖艳松..施用井冈霉素后烟草叶际微生态变化规律[J].农业科技与技术（英文）,2025,26(2):52-59,8.基金项目
Supported by Science and Technology Project of Hengyang Branch of Hunan Tobacco Company(HYYC2024KJ22) 湖南省烟草公司衡阳市公司科技项目(HYYC2024KJ22) （HYYC2024KJ22）
