摘要
Abstract
Objective:To investigate the effects of irisin on oxidized low-density lipoprotein(ox-LDL)induced inflammation and apoptosis of human umbilical vein endothelial cells(HUVECs)by regulating the reactive oxygen species(ROS)/p38 mitogen activated protein kinase(p38 MAPK)signaling pathway.Methods:Cultured HUVECs were divided into control group,ox-LDL group,low-dose irisin group,medium-dose irisin group,high-dose irisin group,Anisomycin group.Cell viability was assessed by MTT assay.Apoptosis was detected by flow cytometry.CFH-DA fluorescent probe was used to detect ROS levels.The expression of P-selectin,intercellular adhesion molecule-1(ICAM-1),vascular endothelial cell adhesion molecule-1(VCAM-1)and E-selectin were detected by ELISA.Western blotting was applied to detect protein expression.Results:ox-LDL exposure markedly decreased HUVEC viability and increased apoptosis rate,ROS positive cells,expresion of P-selectin,ICAM-1,VCAM-1,E-selectin,Bax/Bcl-2,p-p38 MAPK/p38 MAPK,cleaved caspase-3.Irisin decreased HUVEC apoptosis rate,ROS positive cells,expresion of P-selectin,ICAM-1,VCAM-1,E-selectin,Bax/Bcl-2,p-p38 MAPK/p38 MAPK,cleaved caspase-3 in a concentration-dependent manner.These protective effects were abolished by Anisomycin.Conclusion:Irisin can inhibit ox-LDL-induced endothelial inflammation and apoptosis of HUVEC,and its mechanism may be achieved by inhibiting the ROS/p38 MAPK signaling pathway.关键词
鸢尾素/活性氧/p38丝裂原活化蛋白激酶/氧化低密度脂蛋白/人脐静脉内皮细胞/炎症/凋亡Key words
irisin/reactive oxygen species/p38 mitogen-activated protein kinase/oxidized low-density lipoprotein/human umbilical vein endothelial cells/inflammation/apoptosis分类
医药卫生
