农业科技与技术（英文）2025，Vol.26Issue(2)：60-66,7.DOI:10.16175/j.cnki.1009-4229.2025.02.008
我国烟草上一种新的细菌性软腐病病原菌的分离与鉴定
Identification of Bacterial Soft Rot Disease on Tobacco in China
摘要
Abstract
In 2022,an unknown bacterial disease was discovered in tobacco fields of Chenzhou,Hunan Province.In order to identify the pathogen of this disease,this study used tissue isolation method and streak plate method to isolate and purify the pathogen,conducted pathogenicity testing through in-vivo inoculation of Yunyan 87 tobacco plants,and identified the pathogen through morphological observation,physiological and biochemical characterization,and 16S rDNA and gyrB gene sequence analysis.The results indicated that 8 strains were obtained,named BY-1 to BY-8,by isolating the pathogen from infected plants.Pathogenicity test results showed that these isolated strains were the pathogen of the soft rot disease,consistent with Koch's postulates.Combined with morphological observation and physiological and biochemical indicator determination,the strain was preliminarily identified as Pseudomonas.Homology comparison results revealed that the homology similarity of this strain to Pseudomonas flavescens was 99.86%.Phylogenetic analysis was conducted based on 16S rDNA and gyrB genes,and the results indicated that strain BY-8 was clustered with P.flavescens.Therefore,the pathogen was identified as P.flavescens,and the disease was named tobacco bacterial soft rot caused by P.flavescens.This was the first report of this disease on tobacco in China.关键词
烟草细菌性软腐病/病原菌鉴定/黄假单胞菌Key words
Tobacco bacterial soft rot/Pathogen identification/Pseudomonas flavescens引用本文复制引用
黎萍,李思军,王迎,钟双玉,钟杰,肖艳松..我国烟草上一种新的细菌性软腐病病原菌的分离与鉴定[J].农业科技与技术（英文）,2025,26(2):60-66,7.基金项目
Supported by Tobacco Agricultural Science and Technology Innovation Project of Chenzhou Branch of Hunan Tobacco Company(CZYC2024JS05) 湖南省郴州市烟草公司烟草农业科技创新项目(CZYC2024JS05) （CZYC2024JS05）
