解剖学杂志2025，Vol.48Issue(4)：314-318,5.DOI:10.3969/j.issn.1001-1633.2025.04.008
沉默AK035396表达对脂多糖诱导的人脐静脉血管内皮细胞损伤的作用机制
Effect and mechanism of silencing lncRNAAK035396 on lipopolysaccharide-induced damage to human umbilical vein endothelial cells
摘要
Abstract
Objective:To investigate the effects of long non-coding RNA(lncRNA)AK035396 on lipopolysaccharide-induced vascular endothelial cell injury by regulating the miR-217-5p/chloride intracellular channel protein 4(CLIC4)signaling pathway.Methods:Human umbilical vein endothelial cells(HUVECs)were cultured in vitro.An injury model was established through induction of 1 μg/mL lipopolysaccharide.The HUVECs were divided into the control group(infected with lentivirus lenti-si-NC)and the si-AK035396 group(infected with lentivirus lenti-si-AK035396).Quantitative real-time PCR was used to analyze the expression levels of AK035396,miR-217-5p and CLIC4 mRNA.MTT method and flow cytometry were adopted to detect the viability and apoptosis of HUVECs cells respectively.Dual luciferase assay was utilized to verify the targeting relationship between AK035396 and miR-217-5p.Western blotting was applied to detect the expression levels of CLIC4,Caspase-3,Caspase-9,and Bax proteins.Results:Compared with the control group,the expression of AK035396 in HUVECs cells in the si-AK035396 group was reduced.The viability of HUVECs cells in the si-AK035396 group was significantly higher than that in the control group.The apoptosis rate of cells in the si-AK035396 group was significantly lower than that in the control group.AK035396 can directly target and bind to miR-217-5p.Compared with the control group,the expression of miR-217-5p in HUVECs in the si-AK035396 group was significantly increased,with the expression of CLIC4 mRNA significantly decreased,and the proteins of CLIC4,Caspase-3,Caspase-9,and Bax significantly decreased.Conclusion:AK035396 silencing alleviates lipopolysaccharide-induced vascular endothelial cell injury by regulating the miR-217-5p/CLIC4 signaling pathway.关键词
人脐静脉血管内皮细胞/脂多糖/长链非编码RNA/miR-217-5p/细胞内氯离子通道蛋白4/凋亡Key words
human umbilical vein endothelial cells/lipopolysaccharide/long non-coding RNA/miR-217-5p/chloride intracellular channel protein 4/apoptosis分类
医药卫生引用本文复制引用
李红晓,杨阳,刘维,王强..沉默AK035396表达对脂多糖诱导的人脐静脉血管内皮细胞损伤的作用机制[J].解剖学杂志,2025,48(4):314-318,5.基金项目
国家自然科学基金(81960867) （81960867）
