江苏农业学报2025，Vol.41Issue(8)：1457-1465,9.DOI:10.3969/j.issn.1000-4440.2025.08.001
水稻分蘖数遗传位点挖掘与候选基因分析
Identification of genetic loci for rice tiller number and analysis of candi-date genes
摘要
Abstract
Tiller number is a key agronomic trait that directly determines rice yield.Therefore,mining genes related to tiller number is crucial for the genetic mechanism investigation of tiller number and variety im-provement.In this study,186 recombinant inbred lines(RILs)were constructed from japonica rice TD70 and in-dica rice Kasalath,which had obvious differences in tiller number.Data of tiller-number and genotype collected in 2021 and 2023 were jointly analysed with the quantitative trait lo-cus(QTL)IciMapping V3.4 software to identify rice tiller related QTLs by inclusive composite interval mapping method.A total of 17 QTLs related to tiller number were detected on Chromosome One,Chromosome Two,Chromosome Four,Chro-mosome Five,Chromosome Six,Chromosome Seven,Chromosome Eleven and Chromosome Twelve respectively in two years,with logarithm of odds(LOD)scores ranged from 2.66 to 9.14,and the phenotypic variation explained from 1.48%to 8.68%.QTL mapping physical interval analysis revealed that three of the QTLs overlapped or were adjacent to previously reported QTL loci related to tiller number,two of which were identified as tiller number genes.qTN2-1 and qTN4 were de-tected in two years and showed high stability.The qTN2-1 was mapped within 7.12-7.25 Mb on Chromosome Two,the LOD values were 3.96 and 7.14,and the contribution rates were 7.58%and 6.45%in 2021 and 2023,respectively.The qTN4 was mapped within 2.43-2.67 Mb on Chromosome Four,the LOD values were 3.94 and 2.66,and the contribution rates were 6.86%and 8.42%in 2021 and 2023,respectively.Variation analysis of sequences between parents within the mapping intervals of qTN2-1 and qTN4 showed that,there were missense mutations in the coding regions of six genes be-tween parents.The RILs population strains were further classified into HapA and HapB alleles based on the type of single nucleotide polymorphism(SNP).Results of effect showed that,the tiller number showed significant or extremely signifi-cant difference between different alleles.Two candidate genes for tiller number were identified combined with results of ex-pression analysis,which provided theoretical basis for analyzing the molecular mechanism of tiller number in rice and of-fered novel loci for high-yield rice breeding.关键词
水稻/分蘖数/Bin图谱/QTL定位Key words
rice/tiller number/Bin map/QTL mapping分类
农业科技引用本文复制引用
姚林江,赵春芳,路凯,王才林,朱克明,张亚东,梁文化,陈涛,朱镇,赵庆勇,李余生,姚姝,赵凌,周丽慧..水稻分蘖数遗传位点挖掘与候选基因分析[J].江苏农业学报,2025,41(8):1457-1465,9.基金项目
现代农业产业技术体系建设专项(CARS-01-69) （CARS-01-69）
