江苏农业学报2025，Vol.41Issue(8)：1466-1474,9.DOI:10.3969/j.issn.1000-4440.2025.08.002
基于CRISPR/Cas9基因编辑技术改良扬麦18农艺与品质性状
Improvement of agronomic and quality traits of Yangmai 18 using CRISPR/Cas9 gene editing technology
摘要
Abstract
Jiangsu province is an important wheat-producing province in China,spanning the middle and lower reaches of the Yangtze River(MLRYR)and the Huang-Huai region,two major wheat-growing areas.The wheat planted in the MLRYR is mainly red-skinned spring wheat,which usually has large panicles and a loose plant type,and is resistant to Fusarium head blight(FHB),powdery mildew(PM),and pre-harvest sprouting(PHS).In contrast,the wheat planted in the Huang-Huai re-gion is mainly white-skinned winter wheat,which has a compact plant type and a higher number of panicles,but is generally sus-ceptible to FHB,PM,and PHS.To develop new wheat germplasms that meet different application needs,the vernalization-relat-ed gene Vrn-D1,grain hardness-related genes Pina and Pinb,and the seed coat color-related gene Myb10 were edited in the red-skinned spring soft wheat variety Yangmai 18 using CRISPR/Cas9 technology.A total of six Vrn-D1 mutants,five Pin mutants,and seven Myb10 mutants were obtained.Phenotypic identification results showed that compared with the wild type,the second and fourth internode lengths of the Vrn-D1 mutants were significantly reduced(with the exception of the vrnM5 line),the grain hardness of the Pin mutants was sig-nificantly increased,and the seed coat color of five Myb10 mutants changed from red to white.The results of this study provide a reference for using CRISPR/Cas9 gene editing technology to improve specific agronomic and quality traits of wheat.关键词
基因编辑/春化基因/籽粒硬度基因/种皮颜色基因Key words
gene editing/vernalization gene/grain hardness gene/seed coat color gene分类
农业科技引用本文复制引用
赵霈泽,万文涛,赵仁慧,蒋正宁,吴荣林,别同德,陈升位..基于CRISPR/Cas9基因编辑技术改良扬麦18农艺与品质性状[J].江苏农业学报,2025,41(8):1466-1474,9.基金项目
国家自然科学基金项目(32472102) （32472102）
江苏里下河地区农业科学研究所科研专项[SJ(22)114] （22）
