江苏农业学报2025，Vol.41Issue(8)：1499-1507,9.DOI:10.3969/j.issn.1000-4440.2025.08.006
绣球花叶枯病病原菌鉴定、生物学特性及杀菌剂室内毒力测定
Identification and biological characterization of the pathogen causing leaf blight on Hydrangea macrophylla and in vitro toxicity bioassay of fungicides
摘要
Abstract
To identify the pathogen responsible for hydrangea leaf blight,a tissue isolation method was employed on diseased leaf samples of Hydrangea macrophylla Endless Summer collected from Nanjing City,Jiangsu Province,China.Through back-inoculation experiments,strain YK-B was identified as the pathogenic fungus.The study found that inocula-tion of healthy hydrangea leaf tissues with strain YK-B induced leaf blight symptoms consistent with those observed on the originally collected diseased samples.Based on morphological characteristics and multigene phy-logenetic analysis,the pathogenic strain YK-B was identi-fied as Alternaria alternata.Furthermore,mycelial growth experiments were conducted with the pathogenic strain YK-B under varying conditions of carbon sources,nitrogen sources,temperature,and pH.The results indicated that the optimal conditions for mycelial growth of YK-B were raffinose as the carbon source,peptone as the nitrogen source,a temperature of 25℃,and a pH range of 6 to 8.The re-sults of in vitro toxicity assays of seven fungicides against the pathogenic strain YK-B revealed that copper hydroxide exhibi-ted the most effective inhibition.The findings of this study provide practical guidance for the diagnosis and management of hydrangea leaf blight.关键词
绣球花/叶枯病/病原菌鉴定/生物学特性/毒力测定Key words
Hydrangea macrophylla/leaf blight/pathogen identification/biological characteristics/toxicity test分类
农业科技引用本文复制引用
陈慧杰,毛俊丹,邓喆,刘欣童,陈双双,冯景,金玉妍,邓衍明..绣球花叶枯病病原菌鉴定、生物学特性及杀菌剂室内毒力测定[J].江苏农业学报,2025,41(8):1499-1507,9.基金项目
江苏省农业科技自主创新基金项目[CX(2023)3062] （2023）
华东地区花卉生物学国家林业和草原局重点实验室开放基金项目(KFE202401) （KFE202401）
中央财政林业科技推广示范项目[苏(2024)TG03)] （2024）
