临床口腔医学杂志2025，Vol.41Issue(9)：515-520,6.DOI:10.3969/j.issn.1003-1634.2025.09.002
具有成血管潜能的M2巨噬细胞来源外泌体在牙髓再生中的研究初探
A preliminary study of M2 macrophage-derived exosomes with angiogenic potential in dental pulp regenera-tion
摘要
Abstract
Objective:This study was designed to investigate the effects of M2 macrophage-derived exosomes(M2-Exos)on the angiogenic potential of dental pulp stem cells(DPSCs)and human umbilical vein endothelial cells(HUVECs),and to evaluate their proangiogenic competency and application potential in dental pulp regeneration.Methods:M1-Exos and M2-Exos were extracted by differential centrifugation and identified by transmission electron microscope(TEM),nanoparticle tracking analysis(NTA),and western blotting(WB).Next,PKH-26-labeled exosomes were co-cultured with DPSCs and HUVECs to observe uptake efficiency.Subsequently,the effect of M1-Exos and M2-Exos on the migration capability of DPSCs and HUVECs was assessed by transwell migration assay.Finally,angiogenic capacity was evaluated using Matrigel tube for-mation assay.Results:In this study,M1-Exos and M2-Exos meeting exosomal standards were successfully isolated and identi-fied.Meanwhile,the two exosomes could be effectively taken up by DPSCs and HUVECs.In addition,compared to M1-Exos,which significantly inhibited the migration of DPSCs and HUVECs,the number of migrated cells was considerably increased after M2-Exos application(P<0.05).Importantly,in sharp contrast with M1-Exos,M2-Exos not only promoted the endothelial cell differentiation of DPSCs and induced the formation of vascular-like tubular structures,but also remarkably enhanced the an-giogenic potential of HUVECs to generate regular and orderly capillary-like structures(P<0.05).Conclusion:M2-Exos could significantly facilitate the migration of DPSCs and HUVECs in vitro.Furthermore,they induced the differentiation of endothelial cells of DPSCs and promoted the angiogenesis of HUVECs,suggesting that M2-Exos possess desirable proangiogenic property.关键词
再生性牙髓治疗/血运重建/巨噬细胞/外泌体Key words
Regenerative endodontic therapy/Revascularization/Macrophage/Exosome分类
医药卫生引用本文复制引用
王宇洁,汪一帆,段大洁,刘甄苑,胡筱涵,毛靖,石鑫..具有成血管潜能的M2巨噬细胞来源外泌体在牙髓再生中的研究初探[J].临床口腔医学杂志,2025,41(9):515-520,6.基金项目
国家自然科学基金项目(82401074) （82401074）
湖北省教学改革研究项目(HBJG-250012) （HBJG-250012）
湖北省大学生创新基金项目(S202510487620) （S202510487620）
华中科技大学研究生创新基金项目(YCJJ20252415) （YCJJ20252415）
华中科技大学大学生创新基金项目(X202510487134) （X202510487134）
华中科技大学同济医学院附属同济医院科研基金项目(24-2KYC13061-02) （24-2KYC13061-02）
华中科技大学同济医学院第二临床学院教学研究基金项目(2022058) （2022058）
