临床口腔医学杂志2025，Vol.41Issue(9)：524-529,6.DOI:10.3969/j.issn.1003-1634.2025.09.004
LV-miR-378修饰BMSCs复合3D支架在骨缺损中成骨分化的研究
Study on osteogenic differentiation of LV-miR-378-modified BMSCs combined with 3D scaffolds in bone de-fects
摘要
Abstract
Objective:To investigate the osteogenic differentiation effect of miR-378 overexpression modified BMSCs combined with 3D printed deer antler powder/SF/PVA scaffolds in the repair of sheep jawbone defects.Methods:Sheep bone marrow mesenchymal stem cells(BMSCs)were co transfected with miR-378,and the cell proliferation ability was evaluated by CCK-8 assay.The biocompatibility of the scaffold was evaluated by scanning electron microscope.The transfected BMSCs were co-cultured with scaffolds,and 12 healthy sheep were randomly divided into 3 groups(n=4),LV-miR-378-BMSCs scaf-fold group(experimental group),LV-NC-BMSCs stent group(control group)and BMSCs scaffold group(blank group),and at 4,8,and 12 weeks after surgery,gross observation,micro-CT analysis,histological examination and qRT-PCR detection were performed to study the osteogenic differentiation effect of the scaffold.Results:CCK-8 showed that the cell proliferation activi-ty of LV-miR-378 cell group was higher than the other two groups on 7～11 days(P<0.05).Scanning electron microscopy shows that the 3D deer antler powder composite scaffold has good biocompatibility,and LV-miR-378-BMSCs can climb on the scaffold.There were no deaths among the experimental animals in each group after modeling and implanting the scaffold.Mi-cro-CT showed better healing of the defect area in the experimental group at 4 and 12 weeks,with higher bone density values than the other two groups(P<0.05),while there was no significant difference between the control group and blank group(P>0.05).HE,Masson staining showed that the experimental group had more newly generated osteoblasts at each stage,with orderly arrangement of bone trabeculae.At 12 weeks,more mature bone cells were observed to have formed.qRT-PCR showed that the levels of OPN and IGF-1 in the experimental group were higher than those in the other two groups(P<0.01),while there was no significant difference between the control group and blank group(P>0.05).Conclusion:LV-miR-378 modified BMSCs combined with 3D deer antler powder/SF/PVA scaffold can promote bone repair of sheep jawbone defects.关键词
miR-378/羊骨髓间充质干细胞/羊颌骨缺损/成骨分化/鹿角粉支架Key words
miR-378/Sheep bone marrow mesenchymal cells/Sheep jawbone defect/Osteogenic differentiation/Deer antler powder scaffold分类
生物科学引用本文复制引用
吾西坤·吾然,朱甜甜,夏衣旦·艾合买提江,韩祥祯,周琦琪,何惠宇,李征..LV-miR-378修饰BMSCs复合3D支架在骨缺损中成骨分化的研究[J].临床口腔医学杂志,2025,41(9):524-529,6.基金项目
新疆维吾尔自治区科技支疆计划项目(编号:2022E02043) （编号:2022E02043）
